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C-14072

Sigma-Aldrich

Human White Preadipocytes (HWP)

Subcutaneous, 1 million cells per pellet

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biological source

human adipose tissue (subcutaneous or visceral)

packaging

pkg of 1,000,000 cells

morphology

(adipocytes)

application(s)

cell analysis (DNA, RNA, and protein)

shipped in

dry ice

storage temp.

−20°C

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This Item
C-12732C-14073C-12735
vibrant-m

C-14072

Human White Preadipocytes (HWP)

vibrant-m

C-12732

Human White Preadipocytes (HWP)

vibrant-m

C-14073

Human White Preadipocytes (HWP)

vibrant-m

C-12735

Human White Preadipocytes (HWP-c)

morphology

(adipocytes)

morphology

(adipocytes)

morphology

(adipocytes)

morphology

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

storage temp.

−20°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

application(s)

cell analysis (DNA, RNA, and protein)

application(s)

-

application(s)

cell analysis (DNA, RNA, and protein)

application(s)

-

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Adipose Tissue

Application

Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request).Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physical form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service