C1655

Cholera Toxin B subunit

FITC conjugate, lyophilized powder

• Synonym(s): CTB
• MDL number: MFCD02356522
• UNSPSC Code: 12352200
• PubChem Substance ID: 329774902
• NACRES: NA.77

Properties

• conjugate: FITC conjugate
• Quality Level: 200
• form: lyophilized powder
• mol wt: ~12 kDa
• composition: Protein, ~20% Lowry
• extent of labeling: ~1.0 mol FITC per mol protein
• storage temp.: 2-8°C
• SMILES string: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• InChI key: YDXZSNHARVUYNM-UHFFFAOYSA-N

Description

Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:

• to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat[1]
• to stain epididymal sperms of mice[2]
• to stain peritoneal macrophages of mice[3]

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Analysis Note

Activity measured by ELISA using ganglioside GM₁ coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H332,H412
• pcodes: P261 - P271 - P273 - P304 + P340 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable