For testing purposes, this product is prepared in cold deionized water. While PBS has not been tested as a solvent, the enzyme is expected to be soluble. Please see the link below to review the assay protocol for cellulase and hemicellulase:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-cellulase
C1794
Cellulase from Trichoderma sp.
BioReagent, suitable for plant cell culture, 3-10 units/mg solid
Cellulase from Trichoderma sp.
Synonym(s):
β-1,4-glucanase
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5000 UNITS
$194.00
10000 UNITS
$330.00
About This Item
CAS Number:
UNSPSC Code:
10171502
eCl@ss:
32160410
EC Number:
232-734-4
NACRES:
NA.72
MDL number:
Specific activity:
3-10 units/mg solid
Biological source:
fungus (Trichoderma sp.)
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biological source
fungus (Trichoderma sp.)
product line
BioReagent
form
powder
specific activity
3-10 units/mg solid
contains
maltodextrin
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
cell culture | plant: suitable
application(s)
agriculture
greener alternative category
, Enabling
storage temp.
2-8°C
Quality Level
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1 of 4
This Item | C8546 | C2730 | C1184 |
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) - | technique(s) - |
| specific activity 3-10 units/mg solid | specific activity ≥1 unit/mg solid | specific activity ≥700 units/g | specific activity ≥0.3 units/mg solid |
| biological source fungus (Trichoderma sp.) | biological source - | biological source fungus (Trichoderma reesei) | biological source - |
| form powder | form lyophilized powder | form aqueous solution | form powder |
| application(s) agriculture | application(s) - | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
Cellulase (β-1,4-glucanase) is a natural enzyme produced by the filamentous fungi Trichoderma viride which promotes the endohydrolysis of (1→4)-beta-D-glucosidic linkages in cellulose and lichenin. Cellulases are important for processing potential commercial products such as cellulosic ethanol.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
used together
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Rachel A Levin et al.
The Journal of eukaryotic microbiology, 64(5), 588-597 (2017-01-26)
Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular
Binay Bhushan Panda et al.
BMC plant biology, 18(1), 89-89 (2018-05-23)
Poor filling of grains in the basal spikelets of large size panicles bearing numerous spikelets has been a major limitation in attempts to increase the rice production to feed the world's increasing population. Considering that biotechnological intervention could play important
Jin Zhou et al.
Bioresource technology, 100(2), 819-825 (2008-09-06)
To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven
Mari Aidemark et al.
BMC plant biology, 10, 274-274 (2010-12-16)
Alamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane, mitochondria and plastids of cultured plant
Paula Berenstecher et al.
Current biology : CB, 30(16), 3243-3251 (2020-07-04)
Photodegradation of aboveground senescent plant material (plant litter) due to exposure to solar radiation has been identified as a dominant control on carbon (C) loss in semi-arid ecosystems [1], upturning traditional models of C cycling based only on available moisture
Protocols
To standardize an enzymatic assay procedure of cellulase.
Related Content
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Hi, I was wondering how to prepare the enzyme solution? Can I just dilute it on PBS? Thanks
1 answer-
Helpful?
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Hello, what is the Uniprot number or Gene ID for C1794-5KU? Thank you
1 answer-
The Gene ID for Cellulase from Trichoderma sp. (AKA Gliocladium sp.) is GH45. Please see information at the links below:
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