C1805

Sigma-Aldrich

Monoclonal Anti-CD4 antibody produced in mouse

clone Q4120, purified from hybridoma cell culture

Synonym(s):
Anti-CD4 antibody, Mouse monoclonal
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Q4120, monoclonal

form

buffered aqueous solution

mol wt

59 kDa

species reactivity

human

application(s)

flow cytometry: 5 μL using 1 × 106 cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... CD4(920)

General description

Monoclonal Anti-Human CD4 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with CD4-Transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. Cluster of differentiation 4 (CD4) human cell surface glycoprotein (59kDa) belongs to the immunoglobulin superfamily. It is expressed on the helper/inducer T cell subset, which is found on the majority of peripheral blood neutrophils, most cortical and mature medullary thymocytes, microglial cells, dendritic cells and on some malignancies of T cell origin.

Specificity

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Application

Monoclonal Anti-CD4 antibody produced in mouse has been used in indirect immunofluorescent staining.

Biochem/physiol Actions

Cluster of differentiation 4 (CD4) molecule binds to the major histocompatibility complex (MHC) class II molecules during the interaction of CD4+ T cells with antigen presenting cells or with target cells. It also serves as a high affinity cellular receptor for the GP 120 envelope glycoprotein of the human immunodeficiency virus (HIV-1, HIV-2). The CD4 molecule is involved in the adhesion of T-lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins. Lower levels of CD4 have been detected in monocytes, macrophages and granulocytes.

Target description

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Functional epitope analysis of the human CD4 molecule: antibodies that inhibit human immunodeficiency virus type 1 gene expression bind to the immunoglobulin CDR3-like region of CD4.
Benkirane M, et al.
Journal of virology, 69(11), 6898-6903 (1995)
CD4+ T cells: differentiation and functions
Luckheeram RV, et al.
Clinical & Developmental Immunology (2012)
Jason A LaBonte et al.
Journal of virology, 77(12), 6645-6659 (2003-05-28)
T-tropic (X4) and dualtropic (R5X4) human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins kill primary and immortalized CD4(+) CXCR4(+) T cells by mechanisms involving membrane fusion. However, because much of HIV-1 infection in vivo is mediated by M-tropic (R5) viruses...
Expression of CD4 on human peripheral blood neutrophils
Biswas P, et al.
Blood, 101(11), 4452-4456 (2003)
Down regulation of CD4 expression following isolation and culture of human monocytes
Graziani-Bowering GM and Filion LG
Clinical and Diagnostic Laboratory Immunology, 7(2), 182-191 (2000)

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