All Photos(1)

C1990

Sigma-Aldrich

CelLytic Express

NACRES:
NA.56

Quality Level

shipped in

wet ice

storage temp.

−20°C

General description

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

Application

CelLytic Express has been used as a component in lysis buffer for protein purification.
CelLytic Express is optimized for the lysis of E. coli strain BL21. However, it works well with other commons strains such as JM109. CelLytic Express may also be used on other similar bacterial cells. CelLytic Express has been tested with BL21 E. coli cells expressing histidine-tagged and FLAG fusion proteins. It should be compatib with affinity purification of other fusion proteins.
CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

Packaging

25, 10×25 mL in poly bottle

Features and Benefits

  • Efficient and non-denaturing in-culture protein extraction
  • Save time by eliminating cell harvest and clarification steps
  • Preserve biological activity with fewer manipulations
  • Compatible with affinity resins and magnetic beads
  • Ideal for Production Scale Extraction protocols

Preparation Note

CelLytic® Express is supplied ready-to-use. However, additional reagents may be used depending on the nature of the protein to be isolated. These include salts, protease inhibitors, and reducing agents such as dithiothreitol or 2-mercaptoethanol. The additional reagents should be added into the bacterial culture after dissolving the CelLytic Express, and not by adding them to the dry powder.

Storage and Stability

This product ships on wet ice and storage at –20°C is recommended. CelLytic Express, as supplied, is stable for at least two years when stored properly.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
Celatom is a registered trademark of EP Minerals, LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Emmanuelle Szenker et al.
Cell reports, 1(6), 730-740 (2012-07-21)
Discovering how histone variants that mark distinct chromatin regions affect a developmental program is a major challenge in the epigenetics field. To assess the importance of the H3.3 histone variant and its dedicated histone chaperone HIRA, we used an established...
Veronica Nasta et al.
The FEBS journal, 287(11), 2312-2327 (2019-11-15)
During its late steps, the mitochondrial iron-sulfur cluster (ISC) assembly machinery leads to the formation of [4Fe-4S] clusters. In vivo studies revealed that several proteins are implicated in the biosynthesis and trafficking of [4Fe-4S] clusters in mitochondria. However, they do not...
Jiaquan Liu et al.
Journal of molecular biology, 430(22), 4456-4468 (2018-06-05)
DNA mismatch repair (MMR) is a DNA excision-resynthesis process that principally enhances replication fidelity. Highly conserved MutS (MSH) and MutL (MLH/PMS) homologs initiate MMR and in higher eukaryotes act as DNA damage sensors that can trigger apoptosis. MSH proteins recognize...
Jun Liu et al.
Eukaryotic cell, 12(12), 1588-1599 (2013-07-23)
Microtubules are versatile biopolymers that support numerous vital cellular functions in eukaryotes. The specific properties of microtubules are dependent on distinct microtubule-associated proteins, as the tubulin subunits and microtubule structure are exceptionally conserved. Highly specialized microtubule-containing assemblies are often found...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service