C213140
DMT-2′O-Methyl-rC(ac) Phosphoramidite
configured for MerMade
Recommended Products
biological source
non-animal source (no BSE/TSE risk)
Quality Level
product line
Proligo Reagents
assay
≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)
form
powder
mol wt
801.86 g/mol
impurities
≤0.3% mC2 (reversed phase HPLC, Hydrolysate)
≤0.3% mC3 (reversed phase HPLC, DMT-rC(ac)me)
≤0.3% mC4 (reversed phase HPLC, DMT-rC(ac)me-Ac)
≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (redox titration)
≤1.0% mC1 (redox titration, DMT-rC(ac)me-DMT)
≤3% residual Solvent content
color
white to off-white
suitability
conforms to structure for H-NMR
conforms to structure for LC-MS
compatibility
configured for MerMade
storage temp.
2-8°C
InChI
1S/C42H52N5O9P/c1-28(2)47(29(3)4)57(54-26-12-24-43)56-38-36(55-40(39(38)52-8)46-25-23-37(44-30(5)48)45-41(46)49)27-53-42(31-13-10-9-11-14-31,32-15-19-34(50-6)20-16-32)33-17-21-35(51-7)22-18-33/h9-11,13-23,25,28-29,36,38-40H,12,26-27H2,1-8H3,(H,44,45,48,49)/t36-,38-,39?,40-,57?/m1/s1
InChI key
WNWUMIPFLOKTEZ-IXZONTHDSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
- High yield of crude oligonucleotides
- Compatible with DNA synthesis
- Can be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides
- Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
- Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradation
- Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6minutes compared to 90 seconds for DNA monomers)
- 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry
Application
- Diagnostic probes
- Aptamer and ribozyme development
- Mixed 2′O-Methyl-RNA/DNA antisense molecules
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service