C2181

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

MDL number:

Quality Level

biological source

sheep

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

conjugate

CY3 conjugate

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Application

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment−Cy3 antibody produced in sheep has been used:
  • in immunolabeling of Hela cells
  • as secondary antibody in immunocytochemistry of dendritic cells
  • as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
  • as secondary antibody in immunofluorescence staining keratinocyte cell lines
  • in immunohistochemistry of articular cartilage

The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.

Packaging

1 mL in glass bottle

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.
Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Identification of a Novel Keratin 9 Missense Mutation in a Chinese Family with Epidermolytic Palmoplantar Keratoderma.
Xiao H, et al.
Cellular Physiology and Biochemistry, 46(5), 1919-1929 (2018)
Agili-C implant promotes the regenerative capacity of articular cartilage defects in an ex vivo model.
Chubinskaya S, et al.
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA, 46(5), 1-12 (2018)
A Birkmann et al.
Journal of virology, 75(23), 11583-11593 (2001-11-02)
An immunodominant envelope glycoprotein is encoded by the human herpesvirus 8 (HHV-8) (also termed Kaposi's sarcoma-associated herpesvirus) K8.1 gene. The functional role of glycoprotein K8.1 is unknown, and recognizable sequence homology to K8.1 is not detectable in the genomes of...
Antonio F Ribeiro et al.
Scientific reports, 9(1), 11842-11842 (2019-08-16)
Satellite cells (SCs) are the main muscle stem cells responsible for its regenerative capacity. In muscular dystrophies, however, a failure of the regenerative process results in muscle degeneration and weakness. To analyze the effect of different degrees of muscle degeneration...
Visualization of altered replication dynamics after DNA damage in human cells.
Merrick C, et al.
The Journal of Biological Chemistry, 279(19), 20067-20075 (2004)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service