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C-22216

Sigma-Aldrich

Endothelial Cell Growth Medium 2

Basal Medium, phenol red-free, 500 ml

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form

aqueous solution

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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C-39216C-39211C-22211
Endothelial Cell Growth Medium 2 Basal Medium, phenol red-free, 500 ml

C-22216

Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium 2 SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

C-39216

Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium 2 SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

C-39211

Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium 2 Basal Medium, 500 ml

C-22211

Endothelial Cell Growth Medium 2

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

packaging

pkg of 500 ml

packaging

pkg of 1 vial

packaging

pkg of 1 kit

packaging

pkg of 500 ml

General description

Endothelial Cell Basal Medium 2, phenol red-free (supplements sold separately)

Application

Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Endothelial Cell Media 2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Subculture Routine

Click here for more information.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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