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C-23061

Sigma-Aldrich

Skeletal Muscle Differentiation Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

All Photos(1)
Synonym(s):
Primary Cell Culture Media

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

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C-39366C-23060C-23160
Skeletal Muscle Differentiation Medium Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Sigma-Aldrich

C-23061

Skeletal Muscle Differentiation Medium

Skeletal Muscle Differentiation Medium SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Sigma-Aldrich

C-39366

Skeletal Muscle Differentiation Medium

Skeletal Muscle Cell Growth Medium Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Sigma-Aldrich

C-23060

Skeletal Muscle Cell Growth Medium

Skeletal Muscle Cell Growth Medium Kit including Basal Medium and SupplementPack, 500 ml

Sigma-Aldrich

C-23160

Skeletal Muscle Cell Growth Medium

packaging

pkg of 500 ml

packaging

pkg of 1 vial

packaging

pkg of 500 ml

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

General description

Skeletal Muscle Cell Differentiation Medium (Ready-to-use)

Application

The Skeletal Muscle Cell Differentiation Medium is a serum-free medium developed to induce the fusion of skeletal muscle cells to myotubes with typical multinucleated syncytia. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)

Quality

All lots of PromoCell Skeletal Muscle Cell Differentiation Medium is tested for the capacity to fully induce the differentiation of SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Insulin (recombinant human): 10 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

Primary Human Skeletal Muscle Cells and Growth Media

Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service