Used for immunocytochemistry, with Alexa 488 secondary (Invitrogen)
C2456
Anti-Collagen Type I (COL1A1) Antibody
mouse monoclonal, COL-1
Monoclonal Anti-Collagen, Type I antibody produced in mouse
1 of 1 reviewers took part in a promotion
Synonym(s):
Collagen Type 1 Antibody, Collagen Type 1 Antibody - Monoclonal Anti-Collagen, Type I antibody produced in mouse, Collagen Type I Antibody, Type 1 Collagen Antibody
Sign In to View Organizational & Contract Pricing.
Select a Size
100 μL
$552.00
0.2 ML
$763.00
0.5 ML
$1,440.00
About This Item
Conjugate:
unconjugated
Clone:
COL-1, monoclonal
Application:
DB, ELISA (i), IHC (f)
Citations:
199
Skip To
Product Name
Monoclonal Anti-Collagen, Type I antibody produced in mouse, clone COL-1, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
COL-1, monoclonal
contains
15 mM sodium azide
species reactivity
bovine, human, pig, rat, rabbit, deer
technique(s)
dot blot: suitable, immunohistochemistry (frozen sections): 1:2000 using human or other mammalian frozen sections, indirect ELISA: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Col1a1(29393)
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | |||
|---|---|---|---|
| Gene Information rat ... Col1a1(29393) | Gene Information human ... COL3A1(1281) | Gene Information human ... COL24A1(255631) | Gene Information human ... COL5A1(1289) |
| clone COL-1, monoclonal | clone FH-7A, monoclonal | clone 1D8, monoclonal | clone 2F4, monoclonal |
| antibody form ascites fluid | antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| species reactivity bovine, human, pig, rat, rabbit, deer | species reactivity human, rat | species reactivity human | species reactivity human |
General description
Collagen type I contributes maximum to the fibrous protein content in mammals. It is also present in arteries and extracellular matrix. The protein has an elongated cylindrical structure with tapering ends. It possess a left-handed helix with three polyproline II-type polypeptide strands.
Type I collagen [1α(I)]2α2 is distributed throughout the body. This fibrillar collagen is found in dermis, bone, tendon, ligament, dentin, fasciae, sclera, cornea, organ capsules and fibrous cartilage. It appears in tissues as the classically designated collagen fibers which are formed from densely-packed thin striated fibrils with marked variation in diameter. Collagen I is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts.Collagen type Iα1 (COL1A1) is encoded by the gene mapped to human chromosome 17q21.33. It is the most abundant extracellular matrix (ECM) protein in humans. Type 1 collagen is the major structural protein of bone, tendon, skin and cornea. The encoded protein is a heterotrimer consisting of two α1-chains and one α2-chain.
Immunogen
Bovine skin collagen type I
Application
Monoclonal Anti-Collagen Type I may be used for the localization of type I collagen using various immunochemical assays including ELISA, dot blot and immunohistochemistry. A minimum working dilution of 1:2,000 is determined by indirect immunofluorescent staining of human or other mammalian frozen sections.
Monoclonal Anti-Collagen, Type I antibody produced in mouse has been used in:
- immunohistochemistry
- dot blot technique
- indirect immunofluorescence staining
Biochem/physiol Actions
Collagen type I is responsible for mechanical stability, elasticity, strength and toughness, observed in tendons, ligaments, skin, cornea, bone, dentin and many other tissues.
The antibody is reactive with the native (helical) form of collagen type I in ELISA and dot blot. The product is not reactive when tested on thermally denatured molecules. In immunohistochemical staining of acetone-fixed and unfixed frozen sections, a strong staining of connective tissue fibers is seen. Using the dot blot and ELISA techniques, the antibody shows no cross-reactivity with collagen types, II, III, IV, V, VI, VII, IX, X and XI. The epitope recognized by the antibody is sensitive to routine formalin fixation and paraffin embedding.
Type 1 collagen serves a structural role in the extracellular matrix by providing mechanical support and resistance to tension. Some of the more important genetic diseases, directly or indirectly involving this collagen type include the majority cases of osteogenesis imperfecta and certain types of Ehlers Danlos syndrome. The development of antibodies against collagens has provided a powerful method for examining the distribution of these connective tissue proteins and for investigation of epithelial-mesenchymal interactions, tumorigenesis and basement membrane biology in ontogeny and epithelial differentiation.
Physical form
Liquid
Preparation Note
Store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Gene expressions of small leucine-rich repeat proteoglycans and fibulin-5 are decreased in pelvic organ prolapse
Soderberg M W, et al.
Molecular Human Reproduction, 15(4), 251-257 (2009)
Pan Yeung et al.
PloS one, 10(12), e0144139-e0144139 (2015-12-15)
There is a growing trend for researchers to use in vitro 3D models in cancer studies, as they can better recapitulate the complex in vivo situation. And the fact that the progression and development of tumor are closely associated to
Collagen: structure and mechanics, an introduction
Fratzl P.
Collagen: Structure and Mechanics, 1-13 (2008)
Collagen fibrillar structure and hierarchies
Collagen: Structure and Mechanics, 49-80 (2008)
G Orsini et al.
European journal of histochemistry : EJH, 58(4), 2405-2405 (2015-01-13)
Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service