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C2522

Sigma-Aldrich

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥3,000 W-A units/mg protein

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Synonym(s):
Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bovine erythrocytes

Quality Level

form

lyophilized powder

specific activity

≥3,000 W-A units/mg protein

mol wt

30 kDa

pI 

~5.9

storage temp.

−20°C

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This Item
C3640C3934C2624
specific activity

≥3,000 W-A units/mg protein

specific activity

≥2,000 W-A units/mg protein

specific activity

≥2,000 W-A units/mg protein

specific activity

≥3,500 W-A units/mg protein

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

30 kDa

mol wt

-

mol wt

30 kDa

mol wt

-

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

inhibitor

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Protocols

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

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