At a low enough concentration, the DMSO should not hurt the cells. The final concentration of DMSO in the aqueous working medium typically should not exceed 0.1% because higher DMSO concentrations can adversely affect many cultured cells. Dilute the product in the appropriate aqueous medium to provide a physiologically acceptable final concentration.
C2743
Cytochalasin B from Drechslera dematioidea
Ready Made Solution, 10 mg/mL in DMSO
Synonym(s):
Phomin
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About This Item
Empirical Formula (Hill Notation):
C29H37NO5
CAS Number:
Molecular Weight:
479.61
UNSPSC Code:
51102829
NACRES:
NA.85
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1096207
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InChI key
GBOGMAARMMDZGR-TYHYBEHESA-N
SMILES string
C[C@@H]1CCC[C@@H](O)\C=C\C(=O)O[C@]23[C@@H](\C=C\C1)[C@H](O)C(=C)[C@@H](C)[C@H]2[C@H](Cc4ccccc4)NC3=O
InChI
1S/C29H37NO5/c1-18-9-7-13-22(31)15-16-25(32)35-29-23(14-8-10-18)27(33)20(3)19(2)26(29)24(30-28(29)34)17-21-11-5-4-6-12-21/h4-6,8,11-12,14-16,18-19,22-24,26-27,31,33H,3,7,9-10,13,17H2,1-2H3,(H,30,34)/b14-8+,16-15+/t18-,19-,22-,23+,24+,26+,27-,29-/m1/s1
form
ready-to-use solution
concentration
10 mg/mL in DMSO
antibiotic activity spectrum
fungi
mode of action
cell membrane | interferes
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | C2618 | C6762 | B5936 |
|---|---|---|---|
| antibiotic activity spectrum fungi | antibiotic activity spectrum fungi | antibiotic activity spectrum fungi | antibiotic activity spectrum neoplastics |
| mode of action cell membrane | interferes | mode of action enzyme | interferes | mode of action DNA synthesis | interferes | mode of action cell membrane | interferes |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form solution | form solution | form powder | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| concentration 10 mg/mL in DMSO | concentration 5 mg/mL in DMSO | concentration - | concentration 10 mg/mL in DMSO |
Application
Cytochalasin B from Drechslera dematioidea has been used:
- to block cytokinesis, to obtain binucleated cells[1]
- as a specific glucose transporter inhibitor[2]
- to inhibit glucose transport by class I and III GLUTs to determine the 50% inhibitory concentration (IC50) in HCMV-infected and mock-infected cells[3]
Biochem/physiol Actions
Cell permeable fungal toxin that disrupts contractile microfilaments by inhibiting actin polymerization. This, in turn, induces DNA fragmentation, inhibits cell division, and disrupts many cell processes. Inhibits glucose transport.
Cytochalasin B can prevent cytoplasmic division without affecting nuclear division.[7]
One of a group of fungal metabolites that interfere with a wide variety of cellular movements. Useful tool for characterizing some of the polymerization properties of actin, and in studies on cytokinesis. Probe for the two hexose-transport systems in rat L6 myoblasts.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 4 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
185.0 °F - closed cup - Solvent
flash_point_c
85 °C - closed cup - Solvent
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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I have a solution of Product C2743, Cytochalasin B, in DMSO. Is the DMSO toxic to cultured cells?
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Is Product C2743, Cytochalasin B, cell culture grade?
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The product has been sterile filtered, but it has not been cell culture tested by Sigma.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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