Serum-free tumorsphere medium w/o phenol red, e.g. for hormone response-related assays using estrogen/androgen-responsive cells.
Works well for multiple cell lines but make sure to use the right ECM coating! Reproducible performance. Tumorspheres grow well and can be serially passaged (unlimited). Reasonable cost for given performance.
C-28075
3D Tumorsphere Medium XF
Ready-to-use kit including Basal Medium and SupplementMix, phenol red-free, 250 ml
3D Tumorsphere Medium XF
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form
liquid
packaging
pkg of 250 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
1 of 4
This Item | C-22216 | C-28070 | C-22275 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form aqueous solution | form liquid | form aqueous solution |
| packaging pkg of 250 ml | packaging pkg of 500 ml | packaging pkg of 250 ml | packaging pkg of 500 ml |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
General description
3D Tumosphere Medium XF, phenol red-free
Application
The 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.3D Tumorsphere Medium XF consists of a bottle of phenol red-free Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.
Preparation Note
3D Tumorsphere Medium XF contains all growth factors and supplements. The medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.Thaw the SupplementMix at 15 to 25°C. Aseptically mix the supplement solution by carefully pipetting up and down. Then, transfer the entire content of the SupplementMix to the Basal Medium. Close the bottle and swirl gently until a homogenous mixture is formed.
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Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Analysis Note
All lots of the PromoCell 3D Tumorsphere Medium XF are subjected to comprehensive quality control tests using human mammary cancer cells. Each lot of PromoCell 3D Tumorsphere Medium XF is tested for the ability to support tumorsphere/mammosphere formation, proliferation and serial passage of the 3D culture. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage Class
10 - Combustible liquids
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