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C2885

Sigma-Aldrich

CHES

≥99.0% (titration)

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Synonym(s):
2-(Cyclohexylamino)ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
Molecular Weight:
207.29
Beilstein:
2967601
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Assay

≥99.0% (titration)

form

crystalline

pH

3.0-5.0 (103.6 g/L)

useful pH range

8.6-10.0

pKa (25 °C)

9.3

solubility

water: 0.6 g/25 mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

Inchi Key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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This Item
29311C8210C6013
CHES ≥99.0% (titration)

Sigma-Aldrich

C2885

CHES

-
CHES BioUltra, ≥99.5% (T)

Sigma-Aldrich

29311

CHES

Premium Grade
CHES BioXtra, ≥99.0% (titration)

Sigma-Aldrich

C8210

CHES

Premium Grade
form

crystalline

form

powder

form

powder

form

powder

pH

3.0-5.0 (103.6 g/L)

pH

3.0-5.0 (25 °C, 0.5 M in H2O)

pH

3.0-5.0 (0.5 M in H2O)

pH

-

solubility

water: 0.6 g/25 mL, clear, colorless

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

H2O: 0.5 M, clear, colorless

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Application

CHES has been used:
  • as a buffer in mannanase activity assay of antarctic fungal isolates
  • as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
  • as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
Pack Size/Quantity

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Hemicellulase activity of antarctic microfungi
Bradner JR, et al.
Journal of Applied Microbiology, 87(3), 366-370 (1999)
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Langmuir : the ACS journal of surfaces and colloids, 24(18), 10265-10272 (2008-08-20)
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to

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