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C2978

Sigma-Aldrich

CelLytic M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

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Synonym(s):
Whole-cell protein extraction solution
NACRES:
NA.56

Quality Level

form

solution

storage temp.

room temp

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This Item
C1990CE0050CB0500
CelLytic™ M Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

Sigma-Aldrich

C2978

CelLytic M

CelLytic™ Express

Sigma-Aldrich

C1990

CelLytic Express

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Features and Benefits

  • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
  • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
  • Convenient: Ready-to-use reagent requires no scraping from culture plates
  • Fast: Rapid cell lysis at room temperature.

Quantity

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

Analysis Note

Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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The superoxide free radical (O(2)(•-)) has been viewed as a likely major contributor to aging. If this is correct, then superoxide dismutase (SOD), which removes O(2)(•-), should contribute to longevity assurance. In Caenorhabditis elegans, overexpression (OE) of the major cytosolic
Liliana F Iannucci et al.
Scientific reports, 7(1), 2023-2023 (2017-05-19)
Nonalcoholic fatty liver disease (NAFLD) is a major health problem worldwide, and is often associated with lipotoxic injury, defective mitochondrial function, and insulin resistance. Thyroid hormones (THs) are important regulators of hepatic lipid metabolism. Among the THs, diiodothyronine (T
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Journal of gastroenterology and hepatology, 31(10), 1717-1726 (2016-10-30)
The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Both gene
Yanyong Kang et al.
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G-protein-coupled receptors comprise the largest family of mammalian transmembrane receptors. They mediate numerous cellular pathways by coupling with downstream signalling transducers, including the hetrotrimeric G proteins Gs (stimulatory) and Gi (inhibitory) and several arrestin proteins. The structural mechanisms that define
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