MilliporeSigma
All Photos(2)

C2993

Sigma-Aldrich

Anti-CRMP2 antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Collapsin Response Mediator Protein 2, Anti-Dihydropyriminidase Related Protein-2 (DRP2, DRP-2, DHPRP2), Anti-Dihydropyriminidase-like 2 (DPYSL2), Anti-TOAD-64
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~62 kDa

species reactivity

rat, human, mouse

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

western blot: 0.1-0.2 μg/mL using HeLa whole cell lysate and mouse brain extract (S1 fraction)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... DPYSL2(1808)
mouse ... Dpysl2(12934)
rat ... Dpysl2(25416)

General description

Collapsin response mediator proteins CRMPs) consist of a family of cytosolic phosphoproteins expressed in the nervous system and involved in neuronal differentiation and axonal guidance.

Application

Anti-CRMP2 antibody produced in rabbit has been used in:
  • western blotting
  • immunohistofluorescence
  • epifluorescence imaging
  • coimmunoprecipitation

Biochem/physiol Actions

CRMPs are a part of the collapsin/semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. CRMP2 is upregulated during development, and appears to be crucial for axon outgrowth. Glycogen synthase kinase 3 beta (GSK-3b) phosphorylates and inactivates CRMP-2 downstream of the phosphoinositide-3-kinase (PI3K/Akt) pathway, thus regulating neuronal polarity. CRMP2 interacts with tubulin dimers, kinesin-1 and WASP-family verprolin homologous protein 1 (WAVE1) complex to regulate axon outgrowth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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