C3144

Coenzyme A sodium salt hydrate

cofactor for acyl transfer

• Synonym(s): CoA Na₂
• Empirical Formula (Hill Notation): C₂₁H₃₆N₇O₁₆P₃S · xNa⁺ · yH₂O
• CAS Number: 55672-92-9
• Molecular Weight: 767.53 (anhydrous free acid basis)
• EC Number: 259-747-8
• MDL number: MFCD00079020
• UNSPSC Code: 41106305
• PubChem Substance ID: 24892587
• NACRES: NA.51

Properties

• description: cofactor for acyl transfer
• Quality Level: 200
• assay: ≥85% (spectrophotometric assay)
• form: powder
• solubility: H₂O: soluble 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: [Na+].CC(C)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP(O)([O-])=O)n2cnc3c(N)ncnc23)C(O)C(=O)NCCC(=O)NCCS
• InChI key: SYTRWOCXZXQBPW-CLVRNSBASA-M

Description

General description

Coenzyme A (CoA) is an essential cofactor in living systems and is synthesized from pantothenic acid (vitamin B5),[1] The CoA levels in mitochondria and peroxisomes correspond to 2-5 mM and 0.7 mM, respectively. Cytosolic CoA is in the range of 0.05 mM to 0.14 mM[1]

Application

Coenzyme A is suitable for use in:

• gylcerolipid biosynthesis in porcine adipose tissue[2]
• an assay to measure the level of Alpha-methylacyl-CoA racemase (AMACR) in human blood samples using a nanoparticle electrochemical biosensor[3]
• chloramphenicol acetyltransferase (CAT) assay[4][5]
• the synthesis of palmitoyl-CoA, which is required for palmitoylation and activation of proteins for regulated membrane fusion[6]

Biochem/physiol Actions

Coenzyme A (CoA, CoASH, HSCoA) is a coenzyme that facilitates enzymatic acyl-group transfer reactions and supports the synthesis and oxidation of fatty acids. CoA is involved in the mechanisms of a wide variety of enzymes. In the presence of CoASH, organic carboxylic acids form acyl-CoA thioesters, which facilitates enzyme recognition. The acyl-CoA formed from xenobiotic carboxylic acids can add to the compound′s toxicity, which can lead to cellular metabolic dysfunction.[7] It is involved in the oxidation of pyruvate in the Kreb′s cycle. CoA is needed for metabolic events.[8] The bacterial CoA pathway is targeted for antimicrobial development.[8] It mediates acyl group transfer and carbonyl activation. The CoA and its thioester levels are crucial for cellular homeostasis. CoA is also involved in regulating platelet aggregation and vasoconstriction.[1] It acts as an essential cofactor in enzymatic acetyl transfer reactions.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)