C3228

Sigma-Aldrich

CelLytic MT Cell Lysis Reagent

For mammalian tissues

NACRES:
NA.56
Pricing and availability is not currently available.

Quality Level

Application

CelLytic MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Features and Benefits

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Other Notes

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Quality

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Resveratrol Decreases Oxidative Stress by Restoring Mitophagy and Improves the Pathophysiology of Dystrophin-Deficient mdx Mice
Sebori R, et al.
Oxidative Medicine and Cellular Longevity, 2018 (2018)
Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Joseph S Marino et al.
The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a...
Leticia E Camacho et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(2), R101-R109 (2017-05-12)
Intrauterine growth restriction (IUGR) is associated with persistent metabolic complications, but information is limited for IUGR infants. We determined glucose-stimulated insulin secretion (GSIS) and insulin sensitivity in young lambs with placental insufficiency-induced IUGR. Lambs with hyperthermia-induced IUGR (
Terry D Hinds et al.
The Journal of biological chemistry, 291(48), 25179-25191 (2016-10-21)
Non-alcoholic fatty liver disease is the most rapidly growing form of liver disease and if left untreated can result in non-alcoholic steatohepatitis, ultimately resulting in liver cirrhosis and failure. Biliverdin reductase A (BVRA) is a multifunctioning protein primarily responsible for...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.