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C3260

Sigma-Aldrich

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

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Synonym(s):
Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Porcine heart

Quality Level

form

ammonium sulfate suspension

specific activity

≥100 units/mg protein

mol wt

98 kDa ( 49 kDa monomer)

solubility

H2O: soluble 1.0 mg/mL, clear

foreign activity

isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%

storage temp.

2-8°C

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This Item
G9880L7525E2884
form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

specific activity

≥100 units/mg protein

specific activity

≥75 units/mg protein

specific activity

≥200 units/mg protein

specific activity

≥150 units/mg protein (biuret)

mol wt

98 kDa ( 49 kDa monomer)

mol wt

42 kDa

mol wt

-

mol wt

168 kDa

solubility

H2O: soluble 1.0 mg/mL, clear

solubility

-

solubility

-

solubility

-

foreign activity

isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.05%

foreign activity

glutamic-oxalacetic transaminase ≤0.1%, malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06%

foreign activity

-

Application

Citrate Synthase from porcine heart has been used:
  • to inject newt egg for determining its importance in egg activation at fertilization
  • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
  • to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs

Biochem/physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Unit Definition

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.

Preparation Note

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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