MilliporeSigma
All Photos(4)

C3306

Sigma-Aldrich

Calcium chloride dihydrate

for molecular biology, ≥99.0%

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
CaCl2.2H2O, Calcium chloride, Calcium dichloride
Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
Molecular Weight:
147.01
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

Quality Level

Assay

≥99.0%

form

powder

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

foreign activity

DNase, RNase, protease, none detected

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
21097C56701.01867
Calcium chloride dihydrate BioUltra, for molecular biology, ≥99.5% (KT)

Sigma-Aldrich

21097

Calcium chloride dihydrate

Premium Grade
Calcium chloride anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

C5670

Calcium chloride

Essential+ Grade
Calcium chloride dihydrate crystalline, Ph. Eur., JP, USP, FCC, E 509

SAFC

1.01867

Calcium chloride dihydrate

-
assay

≥99.0%

assay

≥99.5% (KT)

assay

≥96.0%

assay

-

form

powder

form

solid

form

powder

form

crystalline, solid

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

mp

772 °C (lit.)

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

500

Application

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Calcium chloride dihydrate for analysis EMSURE® ACS,Reag. Ph Eur

Supelco

1.02382

Calcium chloride dihydrate

Calcium chloride dihydrate extra pure, for table water, FCC, E 509

SAFC

1.72580

Calcium chloride dihydrate

Calcium chloride anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

C5670

Calcium chloride

Calcium chloride

Sigma-Aldrich

C8106

Calcium chloride

Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Hui Sun Leong et al.
Nature communications, 5, 3947-3947 (2014-05-24)
Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in
Zhijian Zhao et al.
Scientific reports, 10(1), 4399-4399 (2020-03-12)
The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of
Bradley T Estes et al.
Nature protocols, 5(7), 1294-1311 (2010-07-03)
The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well
Nina Meyer et al.
Autophagy, 14(10), 1693-1709 (2018-06-26)
In most cases, macroautophagy/autophagy serves to alleviate cellular stress and acts in a pro-survival manner. However, the effects of autophagy are highly contextual, and autophagic cell death (ACD) is emerging as an alternative paradigm of (stress- and drug-induced) cell demise.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service