Calcium chloride dihydrate

for molecular biology, ≥99.0%

Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


for molecular biology




176 °C (dec.) (lit.)


H2O: 294 mg/mL, clear, colorless

foreign activity

DNase, RNase, protease, none detected

SMILES string




InChI key


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Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)


100, 250, 500 g in poly bottle

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.


Exclamation mark

Signal Word


Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Yikun Mei et al.
Frontiers in microbiology, 11, 996-996 (2020-06-26)
Due to the increasing prevalence of pathogenic fungal infections, the emergence of antifungal resistant clinical isolates worldwide, and the limited arsenal of available antifungals, developing new antifungal strategies is imperative. In this study, we screened a library of 1068 FDA-approved...
Metabolic shift underlies recovery in reversible infantile respiratory chain deficiency.
Hathazi, et al.
The Embo Journal, 39, e105364-e105364 (2021)
Marianne T Vestermark et al.
Acta orthopaedica, 82(5), 614-621 (2011-09-08)
Allografts are often used during revision hip replacement surgery for stabilization of the implant. Resorption of the allograft may exceed new bone formation, and instability of the prosthesis can develop. We investigated whether strontium could regulate the imbalance of fast...
Avinash A Kadam et al.
Bioresource technology, 132, 276-284 (2013-02-16)
Pretreatments to sugarcane bagasse (SCB) such as CaCl2, alkali, ammonia, steam and milling showed 91%, 46%, 47%, 42% and 56% adsorption of Solvent Red 5B (SR5B); 92%, 57%, 58%, 56% and 68% adsorption of simulated dyes mixture (SDM), and 86%...

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