While long term stability studies have not been conducted, stock solutions of this material are expected to be stable for at least 6 months, when stored at 2-8°C. Note that product C0663, Acetylcholinesterase from Erythrocytes, a solution prepared in HEPES buffer pH 8.0 w/0.1% TRITON® X-100, is assigned a retest date of approximately 2 years from the date of Quality Release. This item is stored at 2-8°C.
C3389
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type VI-S, lyophilized powder, 200-1,000 units/mg protein
Synonym(s):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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500 UNITS
$105.00
2000 UNITS
$278.00
10000 UNITS
$953.00
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.77
EC Number:
232-559-3
MDL number:
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Product Name
Acetylcholinesterase from Electrophorus electricus (electric eel), Type VI-S, lyophilized powder, 200-1,000 units/mg protein
type
Type VI-S
form
lyophilized powder
specific activity
200-1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
solubility
20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
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1 of 4
This Item | C1057 | C2888 | C1682 |
|---|---|---|---|
| specific activity 200-1,000 units/mg protein | specific activity ≥900 units/mg protein | specific activity ≥1,000 units/mg protein | specific activity ≥1,000 units/mg protein (Lowry) |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt 280 kDa | mol wt tetramer 440 kDa | mol wt 280 kDa | mol wt 64.6 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.) | solubility H2O: soluble 10 mg/mL | solubility Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5) | solubility - |
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Application
Biochem/physiol Actions
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
General description
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Other Notes
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Physical form
Lyophilized powder containing Tris buffer salts
Preparation Note
This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Jingming Gong et al.
Biosensors & bioelectronics, 24(7), 2285-2288 (2008-12-30)
We developed a simple strategy for designing a highly sensitive electrochemical biosensor for organophosphate pesticides (OPs) based on acetylcholinesterase (AChE) immobilized onto Au nanoparticles-polypyrrole nanowires composite film modifid glassy carbon electrode (labeled as AChE-Au-PPy/GCE). Where, the generated Au nanoparticles (AuNPs)
Makar Makarian et al.
Journal of molecular structure, 1247 (2022-03-01)
In an effort to develop new therapeutic agents to treat Alzheimer's disease, a series of donepezil-based analogs were designed, synthesized using an environmentally friendly route, and biologically evaluated for their inhibitory activity against electric eel acetylcholinesterase (AChE) enzyme. In vitro
Gabriela Villalta et al.
Plants (Basel, Switzerland), 10(6) (2021-07-03)
The essential oil (EO) of Salvia leucantha Cav. was isolated by steam distillation of the aerial parts collected in the South of Ecuador. Its physical properties were evaluated and the chemical composition of the oil was determined by GC-MS and
Giuseppe Uras et al.
Frontiers in neuroscience, 15, 691222-691222 (2021-07-20)
Alzheimer's disease is a neurodegenerative disease characterized by disrupted memory, learning functions, reduced life expectancy, and locomotor dysfunction, as a result of the accumulation and aggregation of amyloid peptides that cause neuronal damage in neuronal circuits. In the current study
Acetylcholinesterase, I. Large-scale purification, homogeneity, and amino Acid analysis.
W Leuzinger et al.
Proceedings of the National Academy of Sciences of the United States of America, 57(2), 446-451 (1967-02-01)
Related Content
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How can I store this enzyme after dissolving it in Tris Buffer for long term?
1 answer-
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Could you please provide the Uniprot accession number for this product? Also, in general, it would be very helpful if this information were included for any items in your catalog that are proteins. Thanks!
1 answer-
UniProt number O42275 is for Protein Acetylcholinesterase, Organism Electrophorus electricus (Electric eel) (Gymnotus electricus).
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The bottle mentions the concentration as 200-1000 U/mg. What exact concentration should we consider for calculation purposes?
1 answer-
The exact concentration is listed on the lot specific Certificate of Analysis. Please see the sample certificate of analysis: https://www.sigmaaldrich.com/certificates/COFA/C3/C3389/C3389-BULK________SLCJ8803__.pdf
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