C3389

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type VI-S, lyophilized powder, 200-1,000 units/mg protein

• Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
• CAS Number: 9000-81-1
• Enzyme Commission number: 3.1.1.7 (BRENDA, IUBMB)
• EC Number: 232-559-3
• MDL number: MFCD00081268

Properties

• type: Type VI-S
• Quality Level: 200
• form: lyophilized powder
• specific activity: 200-1,000 units/mg protein
• mol wt: 280 kDa
• composition: Protein, ≥60% biuret
• solubility: 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E^1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Preparation Note

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves