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C3515

Sigma-Aldrich

Catalase from Aspergillus niger

ammonium sulfate suspension, ≥4,000 units/mg protein

Synonym(s):
H2O2:H2O2 oxidoreductase
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

specific activity

≥4,000 units/mg protein

mol wt

tetramer ~250 kDa

shipped in

wet ice

storage temp.

2-8°C

Application

Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.

Packaging

10, 25 mg in glass bottle

Biochem/physiol Actions

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Caution

Freezing of solutions is not recommended.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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