C3617

Sigma-Aldrich

Anti-Casein Kinase 2β antibody, Mouse monoclonal

clone 6D5, purified from hybridoma cell culture

Synonym(s):
Anti-CK2β
MDL number:
NACRES:
NA.44
Pricing and availability is not currently available.

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6D5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa

species reactivity

human, bovine, rat, hamster, mouse

concentration

~2 mg/mL

application(s)

dot blot: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using total cell extract of HepG2 cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CSNK2B(1460)
mouse ... Csnk2b(13001)
rat ... Csnk2b(81650)

General description

Casein Kinase 2 (CK2) is a ubiquitously expressed serine/threonine kinase that has been implicated in various cellular functions such as cell death, cell cycle regulation, and mRNA synthesis. This protein kinase comprises of two catalytic α subunits and two dimeric β subunits that associate to form a heterotetrameric enzyme complex.CK2 is also involved in regulating viral infections
Monoclonal Anti-Casein Kinase 2β antibody is specific for human, bovine, rat, mouse and hamster Casein Kinase 2β (approx. 30 kDa). The antibody epitope lies within amino-acids 123-127 of human CK2β.

Immunogen

recombinant human casein kinase 2β (CK2β). The antibody epitope resides within amino acids 123-127.

Application

Monoclonal Anti-Casein Kinase 2β antibody is suitable for use in indirect immunofluorescence (using A549 cells ). The product can also be used for western blot (2-4 μg/mL using total cell extract of HepG2 cells), microarray, dot blot and indirect ELISA.

Biochem/physiol Actions

Casein Kinase 2β encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha pr

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
N A St-Denis et al.
Cellular and molecular life sciences : CMLS, 66(11-12), 1817-1829 (2009-04-24)
Protein kinase CK2 is a serine/threonine kinase with a multitude of protein substrates. The enzyme is ubiquitously expressed in mammalian cells, where it functions in a variety of cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. While...
Inessa Ermakova et al.
Journal of molecular biology, 330(5), 925-934 (2003-07-16)
Protein kinase CK2 (formerly called: casein kinase 2) is a heterotetrameric enzyme composed of two separate catalytic chains (CK2alpha) and a stable dimer of two non-catalytic subunits (CK2beta). CK2alpha is a highly conserved member of the superfamily of eukaryotic protein...
Wilhelm Ching et al.
Journal of virology, 86(5), 2400-2415 (2011-12-23)
The human adenovirus type 5 (HAdV5) early region 1B 55-kDa protein (E1B-55K) is a multifunctional phosphoprotein playing several critical roles during adenoviral productive infection, e.g., degradation of host cell proteins, viral late mRNA export, and inhibition of p53-mediated transcription. Many...
Marc P Lussier et al.
The European journal of neuroscience, 39(7), 1148-1158 (2014-04-10)
Controlling the density of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) at synapses is essential for regulating the strength of excitatory neurotransmission. In particular, the phosphorylation of AMPARs is important for defining both synaptic expression and intracellular routing of receptors. Phosphorylation is a...

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