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C3640

Sigma-Aldrich

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

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Synonym(s):
Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
CAS Number:
9001-03-0
Enzyme Commission number:
4.2.1.1 (BRENDA, IUBMB)
EC Number:
232-576-6
MDL number:
MFCD00081467
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥2,000 W-A units/mg protein

pI 

~5.4

storage temp.

−20°C

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C2522C2624C3666
Sigma-Aldrich

Sigma-Aldrich

C3640

Carbonic Anhydrase Isozyme II from bovine erythrocytes

Sigma-Aldrich

Sigma-Aldrich

C2522

Carbonic Anhydrase Isozyme II from bovine erythrocytes

Carbonic Anhydrase from bovine erythrocytes ≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

Sigma-Aldrich

C2624

Carbonic Anhydrase from bovine erythrocytes

Sigma-Aldrich

Sigma-Aldrich

C3666

Carbonic Anhydrase Isozyme II from bovine erythrocytes

specific activity

≥2,000 W-A units/mg protein

specific activity

≥3,000 W-A units/mg protein

specific activity

≥3,500 W-A units/mg protein

specific activity

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

-

Quality Level

200

pI 

~5.4

pI 

~5.9

pI 

-

pI 

5.4

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in pathologies such as glaucoma, epilepsy and Parkinson′s disease.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

inhibitor

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Todd S Davidson et al.
The American journal of pathology, 166(3), 729-736 (2005-03-04)
Autoimmune pancreatitis (AIP), a recently defined disease of unknown etiology, is characterized by inflammatory infiltrates in the pancreas with conspicuous involvement of the ducts. The disease clinically manifests in humans as epigastric pain, weight loss, and jaundice. This report describes
Daumantas Matulis et al.
Biochemistry, 44(13), 5258-5266 (2005-03-30)
ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding
Romualdo Talento et al.
Ultrastructural pathology, 37(1), 70-76 (2013-02-07)
Despite progress in the classification of renal cell carcinomas (RCC), a subset of these carcinomas remains unclassified (RCC-U). Patients with RCC-U usually present at a late stage and have a poor prognosis. Several studies have attempted to extract new classifications
Frédéric D Birkhäuser et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 36(2), 102-111 (2013-02-05)
The dendritic cell vaccine DC-Ad-GM·CAIX is an active, specific immunotherapy with the potential of providing a safe and effective therapy against renal cell carcinoma (RCC). Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to
Peter Burghout et al.
Journal of bacteriology, 195(7), 1573-1582 (2013-01-29)
Although carbon dioxide (CO2) is known to be essential for Streptococcus pneumoniae growth, it is poorly understood how this respiratory tract pathogen adapts to the large changes in environmental CO2 levels it encounters during transmission, host colonization, and disease. To
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Protocols

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

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