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C3710

Sigma-Aldrich

Ciliary Neurotrophic Factor human

CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
CNTF
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤325 ng/mL ED50 ((≥ 3.1 x 103 units/mg))

quality

endotoxin tested

mol wt

22.8 kDa

packaging

pkg of 10 and 20 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

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H8416B3795T2815
Stem Cell Factor human SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

H8416

Stem Cell Factor human

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in NSO cells

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥97% (electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

potency

≤325 ng/mL ED50 ((≥ 3.1 x 103 units/mg))

potency

≤200 ng/mL ED50

potency

-

potency

0.04 ng/mL ED50

mol wt

22.8 kDa

mol wt

dimer 35-45 kDa (glycosylated)

mol wt

~27 kDa

mol wt

25 kDa

General description

The CNTF (ciliary neurotrophic factor) gene encodes a cytokine, and is mapped to human chromosome 11q12.1.

Application

Ciliary Neurotrophic Factor human has been used to study its effect on photoreceptor neuroprotection and Muller glial cell proliferation in zebrafish retina. It has also been used to study the effects of intravitreal injection of ciliary neurotrophic factor (CNTF) on photoreceptor injury and BRB (blood-retinal barrier) breakdown in transgenic model.

Biochem/physiol Actions

Ciliary neurotrophic factor (CNTF) was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). The LIF Rβ subunit is also shared by receptors for LIF and OSM. The gp130 subunit is also shared by receptors for LIF, OSM, IL-6, and IL-11. CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat CNTF share ~83% sequence homology and show cross-reactivity in bioactivity.

Physical form

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Analysis Note

The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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CNTF induces photoreceptor neuroprotection and Muller glial cell proliferation through two different signaling pathways in the adult zebrafish retina
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