C3805

Cyclophilin A human

Name: Cyclophilin A human
Brand: SIGMA
Price: 733 USD

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):

CyP

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About This Item

CAS Number:

95076-93-0

EC Number:

5.2.1.8 (BRENDA, IUBMB)

MDL number:

MFCD00130885

UNSPSC Code:

12352204

NACRES:

NA.54

Key Documents

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biological source

human

Quality Level

200

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

buffered aqueous solution

mol wt

20 kDa

concentration

≥0.3 mg/mL

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P62937

storage temp.

−20°C

Gene Information

human ... PPIA(5478)

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This Item	ECM001	SAB4200360	F135
Sigma-Aldrich C3805 Cyclophilin A human	Sigma-Aldrich ECM001 Fibronectin human	Sigma-Aldrich SAB4200360 Anti-Cyclophilin B antibody, Mouse monoclonal	Sigma-Aldrich F135 Fractalkine, Extracellular Domain human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) western blot: 2.5-5.0 μg/mL using whole cell extracts of human HepG2 or rat NRK cells.	technique(s) cell culture \| mammalian: suitable
Gene Information human ... PPIA(5478)	Gene Information human ... FN1(2335)	Gene Information human ... PPIB(5479) mouse ... Ppib(19035) rat ... Ppib(64367)	Gene Information human ... CX3CL1(6376)
assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay -	assay ≥97% (SDS-PAGE)
biological source human	biological source human	biological source mouse	biological source human
recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant -	recombinant expressed in NSO cells
form buffered aqueous solution	form lyophilized powder	form buffered aqueous solution	form lyophilized powder

General description

Cyclophilins are a family of extremely conserved proteins, known as immunophilins. Cyclophilin A (CyPA) is present in all tissues in prokaryotes and eukaryotes. It is present in all organs of human. Cyclophilin A (CyPA), a 20 kDa chaperone protein, that is liberated from vascular smooth muscle cells (VSMCs).[1]

Application

Cyclophilin A human has been used in the study to interpret the mechanisms by which extracellular CyPA initiates endothelial activation.[2]

Biochem/physiol Actions

Cyclophilin A (CyPA) participates in intracellular signalling and protein trafficking. It helps to control other proteins activity. CyPA plays a physiological and pathological role in cardiovascular diseases. Hence it acts as an important biomarker and mediator in several cardiovascular diseases, like vascular stenosis, atherosclerosis and abdominal aortic aneurysms. CyPA can induce the proliferation and inflammatory cell migration of vascular smooth muscle cells (VSMC) in vitro and in vivo.[1]

Cyclophilins are peptidyl prolyl isomerases that catalyze the cis-trans isomerization of X-Pro peptide bonds. They are highly-conserved cytoplasmic enzymes that accelerate protein folding and facilitate HIV infectivity. Cyclosporin A binds to cyclophilin and inhibits its activity. The cyclosporin A-cyclophilin complex binds to calcineurin and inhibits T-cell activation. The structure of human, recombinant cyclophilin is given by Holzman, et al.

Physical form

Composed of amino acids 1-165 plus an N-terminal 20 amino acid His-tag

Solution in 20mM Tris, pH 8.0, containing 20 mM NaCl, 0.5 mM DTT and 10% glycerol

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Cyclophilin A

Satoh K, et al.

Circulation Journal, 74(11), 2249-2256 (2010)

Cyclosporine A normalizes mitochondrial coupling, reactive oxygen species production, and inflammation and partially restores skeletal muscle maximal oxidative capacity in experimental aortic cross-clamping.

Julien Pottecher et al.

Journal of vascular surgery, 57(4), 1100-1108 (2013-01-22)

By binding to cyclophilin D, cyclosporine A (CsA) inhibits mitochondrial permeability transition pore (mPTP) opening and prevents mitochondrial dysfunction and ultimately cell death after ischemia-reperfusion (IR) injury in cardiac muscle. This study tested whether CsA would decrease skeletal muscle oxidative

Both TMEM16F-dependent and TMEM16F-independent pathways contribute to phosphatidylserine exposure in platelet apoptosis and platelet activation.

Roger van Kruchten et al.

Blood, 121(10), 1850-1857 (2013-01-11)

Scott syndrome, a bleeding disorder caused by defective phospholipid scrambling, has been associated with mutations in the TMEM16F gene. The role of TMEM16F in apoptosis- or agonist-induced phosphatidylserine (PS) exposure was studied in platelets from a Scott syndrome patient and

Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs.

Päivi Pihlajamaa et al.

The EMBO journal, 33(4), 312-326 (2014-01-24)

Androgen receptor (AR) binds male sex steroids and mediates physiological androgen actions in target tissues. ChIP-seq analyses of AR-binding events in murine prostate, kidney and epididymis show that in vivo AR cistromes and their respective androgen-dependent transcription programs are highly

Genome-wide analysis of murine renal distal convoluted tubular cells for the target genes of mineralocorticoid receptor.

Kohei Ueda et al.

Biochemical and biophysical research communications, 445(1), 132-137 (2014-02-05)

Mineralocorticoid receptor (MR) is a member of nuclear receptor family proteins and contributes to fluid homeostasis in the kidney. Although aldosterone-MR pathway induces several gene expressions in the kidney, it is often unclear whether the gene expressions are accompanied by

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Cyclophilin A human