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C3886

Sigma-Aldrich

Crystal Violet

Dye content ≥90 %

Synonym(s):
Hexamethylpararosaniline chloride, Methyl Violet 10B, Basic Violet 3, Gentian Violet
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
MDL number:
PubChem Substance ID:

form

powder

composition

Dye content, ≥90%

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

Mode of action

cell membrane | interferes
enzyme | inhibits

antibiotic activity spectrum

fungi

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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Application

Crystal Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Used as a component of CV cytotoxicity assays.

Certificate of Analysis

Certificate of Origin

Seo-Hyun Choi et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(8), 771-785 (2019-07-05)
Overexpressed Solute Carrier Family 16 Member 3 (SLC16A3, also called MCT4) plays a critical role in hypoxic cancer cell growth and proliferation, by expelling glycolysis-derived lactate across the plasma membrane. However, how SLC16A3 expression is regulated, under hypoxic conditions, is...
Federica Francescangeli et al.
Journal of experimental & clinical cancer research : CR, 39(1), 2-2 (2020-01-09)
Quiescent/slow cycling cells have been identified in several tumors and correlated with therapy resistance. However, the features of chemoresistant populations and the molecular factors linking quiescence to chemoresistance are largely unknown. A population of chemoresistant quiescent/slow cycling cells was isolated...
Jae-Hoon Ji et al.
Nucleic acids research, 47(12), 6299-6314 (2019-05-03)
Histone H2AX undergoes a phosphorylation switch from pTyr142 (H2AX-pY142) to pSer139 (γH2AX) in the DNA damage response (DDR); however, the functional role of H2AX-pY142 remains elusive. Here, we report a new layer of regulation involving transcription-coupled H2AX-pY142 in the DDR....
Eleonora Grisard et al.
EBioMedicine, 46, 79-93 (2019-07-16)
Metastatic colorectal cancer (CRC) remains a deadly disease. Identifying locally advanced CRC patients with high risk of developing metastasis and improving outcome of metastatic CRC patients require discovering master regulators of metastasis. In this context, the non-coding part of the...
Yi-Deun Jung et al.
Cancers, 11(3) (2019-03-01)
Excess lactate production due to enhanced aerobic glycolysis is characteristic of malignant cancers, which is also intimately associated with poor cancer prognoses. Although tumor-associated lactate contributes to all major steps in carcinogenesis, its action mechanism remains obscure. To understand the...

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