C3956

Sigma-Aldrich

Anti-c-Myc antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.56

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

~0.5 mg/mL

application(s)

immunocytochemistry: 5-10 μg/mL
immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts
indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells
microarray: suitable
western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.

conjugate

unconjugated

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... MYC(4609)

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General description

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. It is located on chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Anti-c-Myc antibody is used for the detection of the c-Myc tag sequence on c-Myc tagged fusion proteins.This polyclonal antibody is produced in rabbit and recognizes the c-Myc sequence at the N-terminus and C-terminus. C3956 is an Affinity purified antibody, increasing sensitivity in most applications.

Specificity

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Immunogen

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

Application

Anti-c-Myc antibody has been used in western blotting and immunofluorescence.
Antibody is recommended for use in immunoblotting, immunoprecipitation and immunofluorescence.
.

Biochem/physiol Actions

MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP). The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as transcriptional regulator of gene expression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Kevin D Augustijn et al.
Infection and immunity, 75(3), 1116-1128 (2006-12-13)
Macrophage migration inhibitory factor (MIF) is a mammalian cytokine that participates in innate and adaptive immune responses. Homologues of mammalian MIF have been discovered in parasite species infecting mammalian hosts (nematodes and malaria parasites), which suggests that the parasites express...
Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and α-synuclein.
Sarkar S, et al.
The Journal of Biological Chemistry, 282(8), 5641-5652 (2007)
Cysteine-string protein isoform beta (Cspβ) is targeted to the trans-Golgi network as a non-palmitoylated CSP in clonal β-cells.
Boal F, et al.
Biochimica et Biophysica Acta, 1773(2), 109-119 (2007)
FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973.
Baudy AR, et al.
EJNMMI Research, 2(1), 22-22 (2012)
Preaxial polydactyly: interactions among ETV, TWIST1 and HAND2 control anterior-posterior patterning of the limb.
Zhang Z, et al.
Development, 137(20), 3417-3426 (2010)

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