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C-41220

Sigma-Aldrich

DetachKit

3 x 250 ml

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packaging

pkg of 3 × 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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C-41210C-41200C-41202
DetachKit 3 x 250 ml

C-41220

DetachKit

DetachKit 3 x 125 ml

C-41210

DetachKit

DetachKit 3 x 30 ml

C-41200

DetachKit

DetachKit 2 3 x 30 ml

C-41202

DetachKit 2

packaging

pkg of 3 × 250 ml

packaging

pkg of 3 × 125 ml

packaging

pkg of 3 × 30 ml

packaging

pkg of 3 × 30 ml

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

DetachKit-250

Application

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

Components

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

Quality

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

Subculture Routine

Click here for more information.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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