Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
C4129
α-Chymotrypsin from bovine pancreas
Type II, lyophilized powder, ≥40 units/mg protein
Synonym(s):
α-chymotrypsin A and B, alpha-chymotrypsin
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250 MG
$73.54
500 MG
$168.00
1 G
$466.45
10 G
$2,340.00
$73.54
List Price$83.00Save 11%Web-Only Promotion
Available to ship onMay 13, 2025Details
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250 MG
$73.54
500 MG
$168.00
1 G
$466.45
10 G
$2,340.00
About This Item
$73.54
List Price$83.00Save 11%Web-Only Promotion
Available to ship onMay 13, 2025Details
Recommended Products
type
Type II
Quality Level
form
lyophilized powder
specific activity
≥40 units/mg protein
mol wt
25 kDa
composition
protein, ≥85%
color
white to off-white
UniProt accession no.
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Gene Information
cow ... CTRB1(618826)
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Application
The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).[1]
Biochem/physiol Actions
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Unit Definition
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Preparation Note
Produced from 3× crystallized chymotrypsinogen
Analysis Note
Protein determined by E1%/280
Other Notes
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
Application
Product No.
Description
Pricing
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Articles
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Protocols
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
Chromatograms
application for HPLC-
How can solutions of alpha chymotrypsin (Product No. C4129) be stored?
1 answer-
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How do you recommend preparing a solution of Product No. C4129?
1 answer-
It is recommended to reconstitute Product No. C4129 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as a stabilizer, and possibly an activator, of the enzyme.
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What is the pH optimum for the enzyme, Product No. C4129?
1 answer-
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of alpha-chymotrypsin from bovine pancreas, product C4129?
1 answer-
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.
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