MilliporeSigma
All Photos(3)

C4562

Sigma-Aldrich

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse

clone hHCD, ascites fluid

Synonym(s):
Anti-L-CAD, Anti-LCAD, Anti-CDM, Anti-NAG22, Anti-HCAD, Anti-h-CD, Anti-H-CAD
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

hHCD, monoclonal

contains

15 mM sodium azide

species reactivity

sheep, mouse, pig, human, rabbit, bovine

technique(s)

immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
immunoprecipitation (IP): suitable
western blot: 1:4,000 using human uterus extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CALD1(800)
mouse ... Cald1(109624)

Specificity

The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture.

Immunogen

human uterus smooth muscle extract.

Application

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for:
  • immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
  • immunoprecipitation
  • western blot at a dilution of 1:4,000 using human uterus extract
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Melissa F Brereton et al.
PloS one, 8(2), e57451-e57451 (2013-02-26)
Adequate blood flow through placental chorionic plate resistance arteries (CPAs) is necessary for oxygen and nutrient transfer to the fetus and a successful pregnancy. In non-placental vascular smooth muscle cells (SMCs), K(+) channels regulate contraction, vascular tone and blood flow.
E Vardar et al.
Biomaterials, 206, 41-48 (2019-03-30)
Stress urinary incontinence (SUI) is a life changing condition, affecting 20 million women worldwide. In this study, we developed a bioactive, injectable bulking agent that consists of Permacol™ (Medtronic, Switzerland) and recombinant insulin like growth factor-1 conjugated fibrin micro-beads (fib_rIGF-1)
Olga S Plekhanova et al.
Journal of vascular research, 43(5), 437-446 (2006-08-11)
Myofibroblasts are involved in vessel remodeling during the development of hypertension as well as after angioplasty and aortocoronary grafting, but the mechanisms of myofibroblastic phenotypic modulation are not fully elucidated. We assessed the role of urokinase plasminogen activator (uPA) and
Jennifer M Kleinhenz et al.
PloS one, 10(10), e0139756-e0139756 (2015-10-10)
Activation of the nuclear hormone receptor, PPARγ, with pharmacological agonists promotes a contractile vascular smooth muscle cell phenotype and reduces oxidative stress and cell proliferation, particularly under pathological conditions including vascular injury, restenosis, and atherosclerosis. However, pharmacological agonists activate both
Philippe Gomis et al.
Journal of neurosurgery, 102(4), 715-720 (2005-05-06)
The aim of this project was to study the perturbations of four smooth-muscle proteins and an extracellular protein, type I collagen, after subarachnoid hemorrhage (SAH) and to examine the possible preventive effects of dexamethasone. Using a one-hemorrhage rabbit model, the

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