Cyclin A is a nuclear protein during S phase and it binds both cdk2 and cdc2. The availability of monoclonal antibody reacting specifically with cyclin A enables the subcellular detection and localization of cyclin A and the measurement of relative differences in cyclin A levels as a function of cell cycle phase.
The antibody recognizes an epitope present in amino acids 162-433 of bovine cyclin A. No cross-reactivity is detected with other cyclins (cyclin B, D, or E).
recombinant bovine cyclin A.
Monoclonal Anti-Cyclin A antibody produced in mouse is suitable for:
- western blotting at a dilution of 1:1,000 using a mouse NIH 3T3 cell extract
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