MilliporeSigma
All Photos(3)

Documents

C4731

Sigma-Aldrich

Anti-Calnexin antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-CNX, Anti-IP90, Anti-P90
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 90 kDa

species reactivity

canine, rat, mouse, human

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunoprecipitation (IP): 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line
indirect immunofluorescence: 1:200 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line
microarray: suitable
western blot: 1:2,000 using whole cell RIPA lysate of the human hepatocytoma HepG2 cell line

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CANX(821)
mouse ... Canx(12330)
rat ... Canx(29144)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C7617C4606C3237
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

mol wt

antigen 90 kDa

mol wt

antigen ~90 kDa

mol wt

antigen 50 kDa

mol wt

antigen 22 kDa

technique(s)

immunoprecipitation (IP): 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, microarray: suitable, indirect immunofluorescence: 1:200 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, western blot: 1:2,000 using whole cell RIPA lysate of the human hepatocytoma HepG2 cell line

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.

technique(s)

immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, microarray: suitable, western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line

technique(s)

indirect immunofluorescence: 1:500 using mouse fibroblast NIH3T3 cell line, microarray: suitable, western blot: 1:2,000 using whole cell extract of the human umbilical vein endothelial HUVEC cell line and the rat fibroblast Rat-1 cell line

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Calnexin contains a long (461 amino acids) N-terminal Ca2+ -binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) transmembrane segment and an acidic cytosolic domain (96 amino acids).
Calnexin, is a calcium binding type I integral membrane protein present mainly in the endoplasmic reticulum. It plays a pivotal role in quality control processes during protein synthesis and folding. Anti-calnexin antibody (diluted 1: 300) can be used in dual immunofluorescence staining to visualize TG2 transport.

Specificity

Rabbit anti-calnexin antibody reacts specifically with calnexin (90 kDa) of dog, mouse, rat and human.

Immunogen

Synthetic peptide corresponding to the c-terminus of human calnexin (amino acids 573-592) conjugated to KLH.

Application

Anti-Calnexin antibody produced in rabbit has been used in:
  • western blot analysis
  • immunofluorescence
  • dual immunofluorescence staining

Biochem/physiol Actions

Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin-like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. Calnexin has been shown to be associated with several cell surface proteins, including major histocompatibility complex (MHC) class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the endoplasmic reticulum (ER). It also forms complexes with other resident ER proteins involved in Ca2+ -dependent retention of proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Quality control system of the endoplasmic reticulum and related diseases
Wu JC, et al.
Acta biochimica et biophysica Sinica, 38(4), 219-226 (2006)
Calnexin and calreticulin, molecular chaperones of the endoplasmic reticulum
Calreticulin, 49-62 (2003)
STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the ER
Alpy F, et al.
Journal of Cell Science, 126(23), 5500-5512 (2013)
I Wada et al.
The Journal of biological chemistry, 266(29), 19599-19610 (1991-10-15)
GTP phosphorylation of rough microsomes in vitro is limited to four integral membrane proteins. Two of these, a phosphoprotein (pp90) and a phosphoglycoprotein (pgp35) were purified as a complex with two nonphosphorylated membrane glycoproteins, gp25H and gp25L. The authenticity of
Juha M Torkko et al.
Molecular and cellular biology, 35(6), 914-927 (2015-01-07)
The type 1 diabetes autoantigen ICA512/IA-2/RPTPN is a receptor protein tyrosine phosphatase of the insulin secretory granules (SGs) which regulates the size of granule stores, possibly via cleavage/signaling of its cytosolic tail. The role of its extracellular region remains unknown.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service