Skip to Content
MilliporeSigma

C4859

Sigma-Aldrich

Cycloheximide solution

Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture

Synonym(s):

CHX

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

1 ML
$138.00

$138.00


In StockDetails


Request a Bulk Order

Select a Size

Change View
1 ML
$138.00

About This Item

Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
Molecular Weight:
281.35
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.77

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

$138.00


In StockDetails


Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

microbial

Quality Level

description

suitable for testing specifications (CHO C. growth inhibitor)

form

ready-to-use solution

concentration

100 mg/mL in DMSO

solubility

DMSO: soluble

antibiotic activity spectrum

fungi
yeast

mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

InChI key

YPHMISFOHDHNIV-FSZOTQKASA-N

Gene Information

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
18079C7698C1988
form

solution

form

liquid

form

powder

form

powder

Quality Level

300

Quality Level

100

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

DMSO: soluble

solubility

-

solubility

ethanol: soluble, clear to hazy

solubility

ethanol: 50 mg/mL

biological source

microbial

biological source

-

biological source

microbial

biological source

-

description

suitable for testing specifications (CHO C. growth inhibitor)

description

-

description

-

description

-

Specificity

Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Hygroscopic. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–3 of 3 Questions  
  1. What concentration should our working solution of this product have?

    1 answer
    1. In cell culture, cycloheximide is typically used at a concentration ranging from 5-50 µg/mL, depending on the cell type and desired experimental outcome. It is often dissolved in DMSO and added to the cell culture medium. It is important to perform a titration to find the optimal concentration for the specific cell line and experiment.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service