In cell culture, cycloheximide is typically used at a concentration ranging from 5-50 µg/mL, depending on the cell type and desired experimental outcome. It is often dissolved in DMSO and added to the cell culture medium. It is important to perform a titration to find the optimal concentration for the specific cell line and experiment.
C4859
Cycloheximide solution
Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture
Synonym(s):
CHX
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1 ML
$138.00
About This Item
Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
Molecular Weight:
281.35
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.77
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biological source
microbial
Quality Level
description
suitable for testing specifications (CHO C. growth inhibitor)
form
ready-to-use solution
concentration
100 mg/mL in DMSO
solubility
DMSO: soluble
antibiotic activity spectrum
fungi
yeast
mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key
YPHMISFOHDHNIV-FSZOTQKASA-N
Gene Information
human ... FKBP1A(2280), PIN1(5300)
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Related Categories
1 of 4
This Item | 18079 | C7698 | C1988 |
|---|---|---|---|
| form solution | form liquid | form powder | form powder |
| Quality Level 300 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility DMSO: soluble | solubility - | solubility ethanol: soluble, clear to hazy | solubility ethanol: 50 mg/mL |
| biological source microbial | biological source - | biological source microbial | biological source - |
| description suitable for testing specifications (CHO C. growth inhibitor) | description - | description - | description - |
Specificity
Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.
Biochem/physiol Actions
Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Hygroscopic. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.
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Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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