Solutions should be stored in single use aliquots at -20ºC. Frozen aliquots should be stable for at least 6 months.
C4951
Cholesterol-Water Soluble
powder, BioReagent, suitable for cell culture
Cholesterol-Water Soluble
Synonym(s):
Cholesterol–methyl-β-cyclodextrin
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biological source
ovine wool
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 300 mg/mL
shipped in
ambient
storage temp.
−20°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| biological source ovine wool | biological source ovine wool | biological source - | biological source non-animal source |
| solubility H2O: 300 mg/mL | solubility H2O: 0.002 mg/mL, Tween: soluble, ethanol: soluble, serum: soluble | solubility H2O: 60 mg/mL | solubility - |
| shipped in ambient | shipped in ambient | shipped in dry ice | shipped in - |
| form powder | form powder | form solid | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
Application
Cholesterol-Water Soluble has been used:
- in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
- in cytotoxicity assay
- for the incubation of CD4+ T cells with Helicobacter pylori[1]
- to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
- to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
- as a supplement for growth of bacterial culture
- to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
- to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
- for reversible inhibition of proteinase K
Packaging
Package size based on cholesterol
Preparation Note
Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.
Other Notes
Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.
related product
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Cholesterol influences potassium currents in inner hair cells isolated from guinea pig cochlea
Kimitsuki T
Auris, Nasus, Larynx, 44(1), 46-51 (2017)
Barbora Melkes et al.
Naunyn-Schmiedeberg's archives of pharmacology, 389(12), 1289-1300 (2016-09-08)
There are some indications that biased μ-opioid ligands may diversely affect μ-opioid receptor (MOR) properties. Here, we used confocal fluorescence recovery after photobleaching (FRAP) to study the regulation by different MOR agonists of receptor movement within the plasma membrane of
ATR-101, a selective and potent inhibitor of Acyl-CoA acyltransferase 1, induces apoptosis in H295R adrenocortical cells and in the adrenal cortex of dogs
LaPensee CR, et al.
Endocrinology, 157(5), 1775-1788 (2016)
Functional characteristics of Lactobacillus spp. from traditional Maasai fermented milk products in Kenya
Mathara J, et al.
International Journal of Food Microbiology, 126(1-2), 57-64 (2008)
Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2
Glende J, et al.
Virology, 381(2), 215-221 (2008)
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How stable is this product when dissolved in water to 300 mg/mL? Can it be frozen for long-term storage?
1 answer-
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What is the molecular weigth of this specific product? Can you share and add g/mol vale please?
1 answer-
The molecular weight of cholesterol used for this product is 386.65 g/mol. For the calculation of molarity based on cholesterol content, approximately 0.4 mg of the 10 mg vial content is cholesterol. The lot-specific Certificate of Analysis should be checked to find the lot-specific cholesterol content for the received product.
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Hi, When you say it has biphasic solubility, I understand what you mean, but in that, when you refer to the mg, do you mean the mg of cholesterol WITHIN the compound methyl-B-cyclodextrin, or the mg of the entire compound?
1 answer-
The reference to mg pertains to the amount of solid powder used, which is 300 mg of the product per mL of water. It is important to allow the solution to sit undisturbed for one hour after dilution to achieve complete solubilization of the product.
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Is there a minimum or maximum solubility limit for this Cholesterol-Cyclodextrin product?
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Due to the nature of cyclodextrin, the cholesterol-cyclodextrin complex exhibits biphasic solubility. To ensure optimal solubility at low concentrations, it is suggested that stock solutions be prepared at a minimum of 50 mg/mL Note that the optimum suggested stock solution concentration is 100 – 300 mg/mL. This material has not been tested for solubility at concentrations greater than 300 mg/mL.
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How long can the cholesterol powder maintain its potency when store at -20 and 4 degree, respectively?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date are subject to a one-year warranty from the date of shipment. For more information, you may access the Product Dating Information document under ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
More details for product solubility and storage can be found in this link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdfHelpful?
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For how long can the powder be stored at -20C?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the Product Dating Information document under ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Is it appropriate to test MbCD as a vehicle when using this product for cell treatment?
1 answer-
Methyl-beta-cyclodextrin, catalog number C4555, would be an appropriate choice to use as a vehicle treatment. More information can be found at https://www.sigmaaldrich.com/product/sigma/c4555.
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Hay algo que no entiendo respecto a este producto. Se asegura que el contenido es de 40 mg de colesterol por g de producto, sin embargo el envase es de 30 mg, eso quiere decir que la concentración de colesterol en el envase es de 1,2 mg? no queda claro
1 answer-
The current specification for this product is that it contains greater than or equal to 40 mg of cholesterol per gram. Each vial contains 30 mg of cholesterol. Therefore, the total mass of the product will always be less than 750 mg.
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C4951 Cholesterol-Water Soluble 30 mg. Technical Support online shows: The 30 mg package size may contain nearly 1 gram of powder. so, what's the other substance besides the 30 mg of the active ingredients?
1 answer-
The bulk of this material is Methyl-?-cyclodextrin. This component enhances the solubility of Cholesterol in water. There are approximately 40 mg of Cholesterol per gram of product, with the remainder being the cyclodextrin agent. Cyclodextrins are not cell permeable and inert in culture. Please see the link below to review additional information regarding these water-soluble complexes:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdfHelpful?
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CAN CHOLESTEROL POWDER BE DISSOLVED IN Medium of cells (High glucose, 2%fbs, 1% p/s) ?
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The product is tested for solubility in water at 300 mg of the powder per 1 mL. Reconstituting in medium or salt solutions may cause a precipitate and is not recommended. Stock solutions in water may then be filter sterilized and added directly to the culture medium. Note that this product is sold on the basis of the cholesterol content only. The 30 mg package size may contain nearly 1 gram of powder. The specific amount of cholesterol per gram is reported on the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/c4951#product-documentationPlease see the links below to learn more about the water-soluble complexes:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/181/278/c4805inf.pdf
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cyclodextrinsHelpful?
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