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C4963

Sigma-Aldrich

Catalase−polyethylene glycol

lyophilized powder, ~40,000 units/mg protein

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Synonym(s):
PEG-Catalase
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

~40,000 units/mg protein

mol wt

PEG 5,000

composition

Protein, ~50% E405

extent of labeling

~40 mol PEG per mol protein

matrix attachment

secondary amine linkage.

storage temp.

−20°C

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1 of 4

This Item
S9549900921901645
vibrant-m

C4963

Catalase−polyethylene glycol

vibrant-m

901645

Methoxy poly(ethylene glycol) KAT

form

lyophilized powder

form

lyophilized powder

form

crystals

form

powder or solid

mol wt

PEG 5,000

mol wt

5000 Da

mol wt

PEG average Mn 2,000, PLGA Mn 3,000

mol wt

PEG average Mn 20,000, PEG ~20,000 Da

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200, 400

Quality Level

100

Quality Level

-

composition

Protein, ~50% E405

composition

Protein, ≥20% biuret

composition

-

composition

-

Application

Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.

Biochem/physiol Actions

Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.

Packaging

Package size based on protein content

Other Notes

Catalase from bovine liver coupled to methoxy-polyethylene glycol.

Physical form

Contains PEG plus 5% citrate buffer salts

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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