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C5041

Sigma-Aldrich

6-Carboxyfluorescein diacetate

~95% (HPLC)

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Synonym(s):
6-CFDA, 6-Carboxy-di-O-acetylfluorescein
Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
Molecular Weight:
460.39
Beilstein:
67043
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Assay

~95% (HPLC)

Quality Level

form

powder

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

Inchi Key

QMOGCCYGOPYYNT-UHFFFAOYSA-N

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This Item
C0662F7378F3651
6-Carboxyfluorescein ~97% (HPLC)

Sigma-Aldrich

C0662

6-Carboxyfluorescein

Fluorescein diacetate used as cell viability stain

Sigma-Aldrich

F7378

Fluorescein diacetate

form

powder

form

powder

form

powder

form

powder

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange

solubility

acetone: 25 mg/mL

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

Quality Level

100, 200

Quality Level

200

Quality Level

200

Quality Level

200

Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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