C5041

6-Carboxyfluorescein diacetate

~95% (HPLC)

• Synonym(s): 6-CFDA, 6-Carboxy-di-O-acetylfluorescein
• Empirical Formula (Hill Notation): C₂₅H₁₆O₉
• CAS Number: 3348-03-6
• Molecular Weight: 460.39
• Beilstein: 67043
• MDL number: MFCD00036871
• PubChem Substance ID: 24892748
• NACRES: NA.47

Properties

• assay: ~95% (HPLC)
• Quality Level: 100; 200
• form: powder
• solubility: chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1
• InChI: 1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
• InChI key: QMOGCCYGOPYYNT-UHFFFAOYSA-N

Description

Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells.[1][2][3] Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.

Packaging

25 mg in poly bottle

100 mg in glass bottle

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves