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Key Documents

C5348

Sigma-Aldrich

Anti-Corticotropin Releasing Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

CRF Antibody - Anti-Corticotropin Releasing Factor antibody produced in rabbit, Crf Antibody, Anti-CRF

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

frog, canine, sheep, rat, human, mouse, pig, bovine

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue
microarray: suitable
radioimmunoassay: 1:10,000 using 2-125I-His12-CRF, human

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CRH(1392)
mouse ... Crh(12918)
rat ... Crh(81648)

General description

Corticotropin Releasing Factor (CRF), a 41 amino acid neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is derived from a larger 196 amino acids precursor protein (preproCRF). CRF is widely distributed in the brain and peripheral nervous system, with the highest levels in the hypothalamic paraventricular nucleus (PVN).

Specificity

The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine.

Immunogen

synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH.

Application

Anti-Corticotropin Releasing Factor antibody produced in rabbit has been used in immunohistochemistry (1:2000) and radioimmunoassay.

Biochem/physiol Actions

Corticotropin Releasing Factor (CRF) is critical in the regulation of the hypothalamic-pituitary-adrenal gland axis, and autonomic and behavioral responses to stress. CRF effects include changes in motor and cognitive behavior, appetite control and modulation of the immune system. CRF is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. The effects of CRF are mediated through CRF receptor subtypes CRFR1 and CRFR2 (splice variants). CRF binds with a high affinity to CRF-binding protein (CRF-BP), which inactivates CRF, while ligands that dissociate CRF from CRF-BP exhibit cognition-enhancing properties. CRF overproduction has been implicated in anorexia and depression and may lead to Cushing′s syndrome. CRF levels are correlated with Alzheimer′s Disease (AD) pathogenesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Kyle D Ketchesin et al.
Stress (Amsterdam, Netherlands), 20(5), 449-464 (2017-04-25)
Corticotropin-releasing hormone (CRH) is a key regulator of the stress response. This peptide controls the hypothalamic-pituitary-adrenal (HPA) axis as well as a variety of behavioral and autonomic stress responses via the two CRH receptors, CRH-R1 and CRH-R2. The CRH system
A Zacharko-Siembida et al.
Polish journal of veterinary sciences, 16(2), 201-209 (2013-08-27)
Calbindin (CB) is a calcium binding protein playing a role in calcium uptake and anti-apoptotic cellular protection. The expression of CB was immunohistochemically studied in the small intestine of normal and red bean kidney lectin-treated suckling piglets. In the duodenum
S Sasaki-Hamada et al.
Neuroscience, 294, 156-165 (2015-03-17)
The intracerebroventicular administration (i.c.v.) of glucagon-like peptide-2 (GLP-2) had antidepressant-like effects on saline-treated mice in the forced-swim test. The GLP-2 treatment (3 μg, i.c.v.) for 6 days, but not that of imipramine had antidepressant-like effects on adrenocorticotropic hormone (ACTH)-treated mice.
Van A Ortega et al.
Frontiers in neuroscience, 7, 196-196 (2013-11-07)
Corticotropin-releasing factor (CRF), urotensin I (UI) and serotonin (5-HT) are generally recognized as key regulators of the anorexigenic stress response in vertebrates, yet the proximal effects and potential interactions of these central messengers on food intake in salmonids are not
Roeland Buckinx et al.
Frontiers in neuroscience, 5, 54-54 (2011-05-05)
Corticotrophin-releasing factor (CRF) is mainly known for its role in the stress response in the hypothalamic-pituitary-adrenal axis. However, increasing evidence has revealed that CRF receptor signaling has additional peripheral effects. For instance, activation of CRF receptors in the gastrointestinal tract

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