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C5367

Sigma-Aldrich

Monoclonal Anti-Casein Kinase 2α antibody produced in mouse

~2 mg/mL, clone 1AD9, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-CK2α
MDL number:
Pricing and availability is not currently available.

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1AD9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

mouse, rat, human

concentration

~2 mg/mL

application(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using total cell extract of MCF7 cells.

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CSNK2A1(1457)
mouse ... Csnk2a1(12995)
rat ... Csnk2a1(116549)

General description

CK2 is a ubiquitously expressed serine/threonine kinase that has been implicated in various cellular functions such as cell death, cell cycle regulation, and mRNA synthesis. This protein kinase comprises of two catalytic α subunits and two dimeric β subunits that associate to form a heterotetrameric enzyme complex.CK2 is also involved in regulating viral infections . Monoclonal Anti-Casein Kinase 2α antibody is specific for casein kinase 2 α in humans, rats and mice (approx. 35 kDa). The antibody-binding epitope resides within amino acids 319-324 of human CK2α. This epitope is conserved across several species.

Immunogen

human denatured recombinant casein kinase 2α.

Application

Monoclonal Anti-Casein Kinase 2α antibody is suitable for use in immunoprecipitation and western blot assays using human fibroblasts . The product can also be used for indirect ELISA and microarray applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Hacer Türkeri et al.
The FEBS journal, 279(3), 395-409 (2011-11-25)
A plastidic serine/threonine protein kinase, initially named plastid transcription kinase (PTK) has been implicated in phosphorylation and redox control of chloroplast transcription. This kinase was later renamed as chloroplast casein kinase 2 (cpCK2) because of its physical and functional similarity...
Yang Gao et al.
Journal of virology, 82(1), 96-104 (2007-10-26)
Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction...
N A St-Denis et al.
Cellular and molecular life sciences : CMLS, 66(11-12), 1817-1829 (2009-04-24)
Protein kinase CK2 is a serine/threonine kinase with a multitude of protein substrates. The enzyme is ubiquitously expressed in mammalian cells, where it functions in a variety of cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. While...
Inessa Ermakova et al.
Journal of molecular biology, 330(5), 925-934 (2003-07-16)
Protein kinase CK2 (formerly called: casein kinase 2) is a heterotetrameric enzyme composed of two separate catalytic chains (CK2alpha) and a stable dimer of two non-catalytic subunits (CK2beta). CK2alpha is a highly conserved member of the superfamily of eukaryotic protein...

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