C5670

Calcium chloride

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

• Synonym(s): CaCl₂, Calcium dichloride
• Linear Formula: CaCl₂
• CAS Number: 10043-52-4
• Molecular Weight: 110.98
• EC Number: 233-140-8
• MDL number: MFCD00010903
• PubChem Substance ID: 24892577
• NACRES: NA.71

Properties

• grade: anhydrous
• vapor pressure: 0.01 mmHg ( 20 °C)
• product line: BioReagent
• Assay: ≥96.0%
• form: powder
• technique(s): cell culture | insect: suitable; cell culture | plant: suitable
• mp: 772 °C (lit.)
• SMILES string: [Cl-].[Cl-].[Ca++]
• InChI: 1S/Ca.2ClH/h;2*1H/q+2;;/p-2
• Inchi Key: UXVMQQNJUSDDNG-UHFFFAOYSA-L

Description

General description

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.^1,2 Extensive reviews of the experimental measurement of biological calcium have been published.^3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.⁵ The CaCl₂-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.⁶ A protocol for the concentration of virus vectors that uses CaCl₂ has been published.⁷CaCl₂ has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.⁸ The crystallization of porcine pancreatic elastase in the presence of CaCl₂ in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.⁹ The effect of CaCl₂ on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.¹⁰

Application

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

Preparation Note

This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable