C5753

Chlorophyll a

from spinach

• Empirical Formula (Hill Notation): C₅₅H₇₂MgN₄O₅
• CAS Number: 479-61-8
• Molecular Weight: 893.49
• Beilstein/REAXYS Number: 3586237
• EC Number: 207-536-6
• MDL number: MFCD00079050
• UNSPSC Code: 12171506
• PubChem Substance ID: 24892803
• NACRES: NA.47

Properties

• biological source: spinach
• Quality Level: 200
• assay: ≥85% (HPLC)
• form: powder
• impurities: chlorophyll b, essentially free
• ε (extinction coefficient): 10.6-11.8 × 10⁴ at 428 nm in diethyl ether (vs. 11.17 × 10⁴, lit.); 8.2-9.1 × 10⁴ at 662 nm in diethyl ether (vs. 8.63 × 10⁴, lit.)
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67
• InChI: 1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1
• InChI key: ATNHDLDRLWWWCB-AENOIHSZSA-M

Description

General description

Chlorophyll a is an important light-harvesting, green or blue color pigment present in the photosynthetic membranes of the chloroplasts in plant cells. It functions as an antenna pigment in photosystems I and II.

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).

Chlorophyll a has been used:

• to study the variation in carotenoids composition with changing colors and maturation in soybean seeds
• to examine the use of chlorophyll distribution maps obtained from hyperspectral imaging for the non-destructive diagnosis of Nitrogen deficiency
• as a standard to determine the concentration of the analyte by fluorescence spectrophotometric technique in the water sample collected from the yellow river
• as a standard to quantify the amount of chlorophyllous pigments gathered from algae by high performance liquid chromatographic (HPLC) technique
• in the identification of pheophytin a from environmentally friendly cultivated green peppers
• to sense different nitric oxide (NO) species

Biochem/physiol Actions

Chlorophyll a binds to the protein molecules CP47 and CP43, forming the inner antenna of the reaction center of Photosystem II. In photosystem I, the binding sites of chlorophyll a includes the polypeptides spread across the membranes. Scarcity in formation of chlorophyll and a low chlorophyll density can lead to deficiency of nitrogen in plants leaves.

Packaging

Sealed ampule.

Safety Information

• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)