We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.
C5914
Cell Dissociation Solution Non-enzymatic 1x
Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Dissociation Solution
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sterility
sterile-filtered
product line
BioReagent
form
liquid
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
6.9-7.1
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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This Item | C5789 | T3449 | S-004-M |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form liquid | form solution | form aqueous solution | form liquid |
| concentration 1 × | concentration 1 × | concentration 1 × | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in - |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. - |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
General description
Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.[1]
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
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Can one use Product C5914, Cell Dissociation Solution, with tissue?
1 answer-
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Does Product C5914, Cell Dissociation Solution, work with epithelial cells?
1 answer-
It will work with most cell types.
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When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?
1 answer-
No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.
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Does Product C5914, Cell Dissociation Solution, contain EDTA?
1 answer-
Yes, it does contain EDTA; the concentration is proprietary.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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