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C5992

Sigma-Aldrich

Anti-Cytokeratin, pan antibody, Mouse monoclonal

clone PCK-26, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-pan-Cytokeratin, Anti-Cytokeratin, Anti-Cytokeratin - Monoclonal Anti-Cytokeratin, pan antibody produced in mouse
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PCK-26, monoclonal

form

buffered aqueous solution

species reactivity

human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: 10-20 μg/mL using human placenta or skin
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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1 of 4

This Item
C1801C2931F3418
species reactivity

human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig

species reactivity

human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

Gene Information

bovine ... Krt1(100301161)
dog ... Krt1(444857)
human ... KRT1(3848), KRT1(3848), KRT1(3848), KRT5(3852), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856), KRT8(3856)
mouse ... KRT1(16678), KRT1(16678), KRT1(16678), Krt1(16678), Krt5(110308), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt2-5(369017), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626), Krt2-8(25626)

Gene Information

bovine ... Krt1(100301161)
dog ... Krt1(444857)
human ... KRT1(3848), KRT1(3848), KRT1(3848), KRT5(3852), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856), KRT8(3856)
mouse ... KRT1(16678), KRT1(16678), KRT1(16678), Krt1(16678), Krt5(110308), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt2-5(369017), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626), Krt2-8(25626)

Gene Information

bovine ... Krt1(100301161)
human ... KRT1(3848), KRT13(3858), KRT13(3858), KRT18(3875), KRT18(3875), KRT4(3851), KRT4(3851), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856)
mouse ... Krt1(16678), Krt10(16661), Krt10(16661), Krt13(16663), Krt13(16663), Krt18(16668), Krt18(16668), Krt4(16682), Krt4(16682), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt1-18(706059), Krt1-18(706059), Krt10(450225), Krt10(450225), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626)

Gene Information

bovine ... Krt1(100301161)
human ... KRT1(3848), KRT13(3858), KRT13(3858), KRT18(3875), KRT18(3875), KRT4(3851), KRT4(3851), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856)
mouse ... Krt1(16678), Krt10(16661), Krt10(16661), Krt13(16663), Krt13(16663), Krt18(16668), Krt18(16668), Krt4(16682), Krt4(16682), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt1-18(706059), Krt1-18(706059), Krt10(450225), Krt10(450225), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

clone

PCK-26, monoclonal

clone

PCK-26, monoclonal

clone

C-11, monoclonal

clone

C-11, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

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General description

Monoclonal Anti-Pan Cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation from human epidermis. Cytokeratins are a group of at least 29 different proteins. They are characteristic of epithelial and trichocytic cells. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and is modified post translationally in terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Specificity

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Immunogen

cytokeratin from human epidermis.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-Cytokeratin, pan antibody produced in mouse has been used in:
  • immunoblotting
  • immunohistochemistry
  • Immunocytochemistry
  • dot blotting

Biochem/physiol Actions

Cytokeratin facilitates the typing of normal, metaplastic, and neoplastic cells and may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas, and neural tumors. It is also useful in detecting micrometastases in lymph nodes and other tissues, and for determining the origin of poorly differentiated tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Derivation and Characterization of Induced Pluripotent Stem Cells from Equine Fibroblasts
Breton A, et al.
Stem Cells and Development, 22(4), 611-611 (2013)
Alison Min-Yan Cheung et al.
Breast cancer research : BCR, 23(1), 114-114 (2021-12-20)
The extent of cellular heterogeneity in breast cancer could have potential impact on diagnosis and long-term outcome. However, pathology evaluation is limited to biomarker immunohistochemical staining and morphology of the bulk cancer. Inter-cellular heterogeneity of biomarkers is not usually assessed.
Marta Campillo Poveda et al.
STAR protocols, 4(4), 102608-102608 (2023-09-26)
Parasitic helminth worms frequently infect the gastrointestinal tract and interact with the intestinal epithelium and specialized cell types within it. Intestinal organoids derived from stem cells that line the intestine represent a transformational technology in the study of epithelial-parasite dialogue.
Ricardo Chaves Vilela et al.
Memorias do Instituto Oswaldo Cruz, 106(6), 701-704 (2011-10-21)
Trichomonas vaginalis and Tritrichomonas foetus are human and bovine parasites, respectively, that provoke the sexually transmitted disease trichomoniasis. These extracellular parasites adhere to the host epithelial cell surface. Although mucinases and proteases have been described as important proteins for parasite
Lina Sun et al.
Scientific reports, 5, 14871-14871 (2015-10-09)
Thymic epithelial cells (TECs) form a 3-dimentional network supporting thymocyte development and maturation. Besides epithelium and thymocytes, heterogeneous fibroblasts are essential components in maintaining thymic microenvironments. However, thymic fibroblast characteristics, development and function remain to be determined. We herein found

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