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C6144

Sigma-Aldrich

Chlorophyll a

from Anacystis nidulans algae

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Empirical Formula (Hill Notation):
C55H72MgN4O5
CAS Number:
Molecular Weight:
893.49
Beilstein:
3586237
EC Number:
MDL number:
PubChem Substance ID:

biological source

Anacystis nidulans algae

form

powder

impurities

chlorophyll b, free

mp

150-153 °C

solubility

diethyl ether: 0.1 mg/10 mL

ε (extinction coefficient)

11.1-12.1 × 104 at 428-430 nm in diethyl ether
8.3-9.4 × 104 at 660-662 nm in diethyl ether

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67

InChI

1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1

InChI key

ATNHDLDRLWWWCB-AENOIHSZSA-M

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This Item
1995832198088390
Chlorophyll a from Anacystis nidulans algae

Sigma-Aldrich

C6144

Chlorophyll a

Lissamine™ Green B Dye content 60 %

Sigma-Aldrich

199583

Lissamine Green B

Cardiogreen for microscopy

Sigma-Aldrich

21980

Cardiogreen

Thiazole Yellow G for microscopy (fluorescence indicator), adsorption indicator

Sigma-Aldrich

88390

Thiazole Yellow G

mp

150-153 °C

mp

-

mp

-

mp

-

solubility

diethyl ether: 0.1 mg/10 mL

solubility

H2O: 0.1%

solubility

water: 0.01 g/10 mL

solubility

hot water: 0.1 g/10 mL

ε (extinction coefficient)

11.1-12.1 × 104 at 428-430 nm in diethyl ether, 8.3-9.4 × 104 at 660-662 nm in diethyl ether

ε (extinction coefficient)

≥10000 at 300-306 nm in water at 0.005 g/L, ≥50000 at 236-242 nm in water at 0.005 g/L, ≥60000 at 630-636 nm in water at 0.005 g/L

ε (extinction coefficient)

≥115 at 386-396 nm in water, ≥2000 at 774-784 nm in water, ≥900 at 712-722 nm in water

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).
Chlorophyll a has been used as a standard for the calibration of fluorometer.

Biochem/physiol Actions

Chlorophyll a is an essential pigment in oxygenic photosynthesis. In photosystems, it is present as an antenna pigment as well as in reaction center I and II. Chlorophyll a is a blue-green pigment which absorbs light in 400-450nm and 640-660nm range. In photosynthesis, the energy absorbed by chlorophyll transforms carbon dioxide and water into carbohydrates and oxygen. When chlorophyll absorbs light energy, an electron in chlorophyll is excited from a lower energy state to a higher energy state. In this higher energy state, the electron is more readily transferred to another molecule. This starts a chain of electron-transfer steps, which ends with an electron transferred to carbon dioxide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
Sealed ampule.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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