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C6158

Sigma-Aldrich

Crystal Violet

ACS reagent, ≥90.0% anhydrous basis

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Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

ACS reagent

Quality Level

Assay

≥90.0% anhydrous basis

form

powder

loss

≤7.5% loss on drying

mp

205 °C (dec.) (lit.)

solubility

water: soluble 50 g/L at 27 °C

density

1.19 g/cm3 at 20 °C

absorption

passes test

suitability

passes test for sensitivity as indicator

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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1 of 4

This Item
C077561135G2039
Crystal Violet ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

C6158

Crystal Violet

Crystal Violet certified by the Biological Stain Commission

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Sigma-Aldrich

61135

Crystal Violet

Gentian Violet meets USP testing specifications

Sigma-Aldrich

G2039

Gentian Violet

assay

≥90.0% anhydrous basis

assay

-

assay

-

assay

-

form

powder

form

powder

form

powder

form

-

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

solubility

water: soluble 50 g/L at 27 °C

solubility

H2O: 1 mg/mL

solubility

-

solubility

-

density

1.19 g/cm3 at 20 °C

density

1.19 g/cm3 at 20 °C

density

1.190 g/cm3

density

-

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Downregulation of survivin expression exerts antitumoral effects on mouse breast cancer cells in vitro and in vivo.
Ma WH, et al.
Oncology Letters, 11, 159-159 (2016)
Plant Chromosomes (1999)
The activation of c-Jun NH2-terminal kinase is required for dihydroartemisinin-induced autophagy in pancreatic cancer cells.
Jia G, et al.
Journal of Experimental & Clinical Cancer Research, 33, 8-8 (2014)
Carson S and Robertson D
Manipulation and Expression of Recombinant DNA (2005)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)

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