MilliporeSigma
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C6164

Sigma-Aldrich

Cell Freezing Medium-DMSO 1×

sterile-filtered, suitable for cell culture

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Synonym(s):
cryopreservation medium
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

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This Item
C6295C9249C6039
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).

Application

Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Antonia Patsialou et al.
Cancer research, 69(24), 9498-9506 (2009-11-26)
Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased
Safety and immunogenicity of oral microencapsulated enterotoxigenic Escherichia coli surface antigen 6 (CS6) with and without LTR192G: a randomized clinical trial
Lapa JA, et al.
Clinical and Vaccine Immunology : CVI (2008)
Variations of components of the plasminogen activation system with the cell cycle in benign prostate tissue and prostate cancer
Plas E, et al.
Cytometry, 46(3), 184-189 (2001)
Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Bahari L, et al.
Testing, 13(2), e0192265-e0192265 (2018)
Scott A Jackson et al.
BMC genomics, 12, 349-349 (2011-07-08)
The gene content of a diverse group of 183 unique Escherichia coli and Shigella isolates was determined using the Affymetrix GeneChip® E. coli Genome 2.0 Array, originally designed for transcriptome analysis, as a genotyping tool. The probe set design utilized

Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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