Documents

C6615

Sigma-Aldrich

Anti-phospho-c-Kit (SCFR) (pTyr730) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-phospho-SCF Receptor (pTyr730)
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, human, mouse

application(s)

western blot: 0.1-1.0 μg/mL using M07e cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... KIT(3815)
mouse ... Kit(16590)
rat ... Kit(64030)

General description

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; c-Kit (Stem cell factor receptor, SCF R; CD117) being the most critical. The signalling triggered by c-Kit results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by c-Kit include Akt, mTOR, MAPK, Sos-Grb2 and c-Jun. Phosphorylation of tyrosine 730 is required for the binding of PLCγ leading to activation of various signalling pathways In addition to development of mast cells, c-Kit also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
The antibody detects human c-Kit pTyr730. Mouse, rat and cow (100% homologous), dog (92% homologous), chicken (85%) are expected to react.

Immunogen

synthetic phosphopeptide derived from the region of human c-Kit that contains tyrosine 730.

Application

Anti-phospho-c-Kit (SCFR) pTyr730 antibody may be used for immunoblotting at a working concentration of 0.1-1.0 μg/ml using M07e cells.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Robert Roskoski
Biochemical and biophysical research communications, 337(1), 1-13 (2005-09-01)
Signaling by stem cell factor and Kit, its receptor, plays important roles in gametogenesis, hematopoiesis, mast cell development and function, and melanogenesis. Moreover, human and mouse embryonic stem cells express Kit transcripts. Stem cell factor exists as both a soluble...
M Tsai et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(14), 6382-6386 (1991-07-15)
We investigated the effects of a newly recognized multifunctional growth factor, the c-kit ligand stem cell factor (SCF), on mouse mast cell proliferation and phenotype. Recombinant rat SCF164 (rrSCF164) induced the development of large numbers of dermal mast cells in...
Dean D Metcalfe
Blood, 112(4), 946-956 (2008-08-08)
Mast cells have been recognized for well over 100 years. With time, human mast cells have been documented to originate from CD34+ cells, and have been implicated in host responses in both innate and acquired immunity. In clinical immunology, they...
Shoko Iwaki et al.
The Journal of biological chemistry, 280(48), 40261-40270 (2005-09-24)
Stem cell factor (SCF) acts in synergy with antigen to enhance the calcium signal, degranulation, activation of transcription factors, and cytokine production in human mast cells. However, the underlying mechanisms for this synergy remain unclear. Here we show, utilizing bone...
Mi-Sun Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 180(7), 4586-4595 (2008-03-21)
Little is known about the signals downstream of PI3K which regulate mast cell homeostasis and function following FcepsilonRI aggregation and Kit ligation. In this study, we investigated the role of the mammalian target of rapamycin complex 1 (mTORC1) pathway in...

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