MilliporeSigma
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C6680

Sigma-Aldrich

Anti-HNK-1/N-CAM (CD57) antibody, Mouse monoclonal

clone VC1.1, purified from hybridoma cell culture

Synonym(s):
Hnk-1 Antibody - Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse, Anti-N-CAM, Anti-CD57, Monoclonal Anti-HNK-1, Hnk-1 Antibody
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

VC1.1, monoclonal

form

buffered aqueous solution

species reactivity

rat, human, feline, mouse

technique(s)

flow cytometry: 5 μL using 1 × 106 cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgM

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

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General description

Mouse Monoclonal Anti-Human CD57/HNK-1 (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a homogenate of cat cerebral cortical area 17. Human natural killer-1 (HNK-1) also called β-1,3-glucuronyltransferase (B3GAT1), is mapped to human chromosome 11q25 and belongs to the glucuronyltransferase gene family. CD57/ HNK-1 is expressed on a subpopulation (15-20%) of peripheral blood mononuclear cells. Expression of the antigen was described for about 60% of NK active cells and for a T-cell subset.

Specificity

Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67

Immunogen

A homogenate of cat primary visual cortex (area 17)

Application

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunostaining and microscopy
  • immunocytochemistry
  • immunofluorescence

Biochem/physiol Actions

Human natural killer-1 (HNK-1)/cluster of differentiation (CD57) is also found on some B-cell lines and B lymphocytic leukemia (B-CLL) melanoma lines. The antigen is also detected in lymphoid tissues, certain neuronal subsets, peripheral nerve Schwann cells, and normal, benign, and malignant prostate. Neurofibromas, schwannomas, traumatic neuromassmall cell lung carcinomas frequently express HNK-1. CD57/HNK-1 appears to be expressed in many species ranging from insects to humans. It is thought to be involved in cellular adhesion events and is expressed on several adhesion related proteins including most neural immunoglobulins superfamily molecules.

Target description

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

Immunohistological analysis of CD1a+ langerhans cells and CD57+ natural killer cells in healthy and diseased human gingival tissue: A comparative study
Stelin S, et al.
Journal of Indian Society of Periodontology, 13(3), 150-150 (2009)
Michelle Ware et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 244(10), 1202-1214 (2015-08-01)
The early axon scaffold is the first axonal structure to appear in the rostral brain of vertebrates, paving the way for later, more complex connections. Several early axon scaffold components are conserved between all vertebrates; most notably two main ventral
A robust method to derive functional neural crest cells from human pluripotent stem cells
Kreitzer FR, et al.
American Journal of Stem Cells, 2(2), 119-119 (2013)
Prognostic role of tumor-infiltrating CD57-positive lymphocytes in solid tumors: a meta-analysis
Hu G and Wang S
Oncotarget, 9(8), 8111-8111 (2018)
A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis
Dubaissi E, et al.
Development, 141(7), 1514-1525 (2014)

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