C6745

Sigma-Aldrich

Collagen Type IV from human cell culture

Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

human cell culture

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mL

concentration

0.3 mg/mL

application(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COL4A1(1282)

Related Categories

General description

COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats . It is an important constituent of type IV collagen in the basement membrane . The COL4A1 gene is located on human chromosome 13q34 .
Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.

Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.

Application

Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation. It has also been used as membrane coatings.

Biochem/physiol Actions

COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Epidemiologically and clinically relevant Group B Streptococcus isolates do not bind collagen but display enhanced binding to human fibrinogen.
Dramsi S, et al.
Microbes and Infection, 14(12), 1044-1048 (2012)
Telomere length defines the cardiomyocyte differentiation potency of mouse induced pluripotent stem cells.
Aguado T, et al.
Stem Cells, 35(2), 362-373 (2017)
Novel mutations in three families confirm a major role of COL4A1 in hereditary porencephaly.
Breedveld G, et al.
Journal of medical Genetics, 43(6), 490-495 (2006)
A severe pulmonary complication in a patient with COL4A1-related disorder: A case report.
Abe Y, et al.
European Journal of Medical Genetics, 60(3), 169-171 (2017)
Association of COL4A1 gene polymorphisms with cerebral palsy in a Chinese Han population.
Bi D, et al.
Clinical Genetics, 90(2), 149-155 (2016)
Articles
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
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