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C6767

Sigma-Aldrich

Congo Red

Dye content ≥35 %

Synonym(s):
Congo Red 4B, Cosmos Red, Cotton Red B, Cotton Red C, Direct Red 28, Direct Red R, Direct Red Y
Empirical Formula (Hill Notation):
C32H22N6Na2O6S2
CAS Number:
Molecular Weight:
696.66
Colour Index Number:
22120
Beilstein:
3894858
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

composition

Dye content, ≥35%

technique(s)

titration: suitable

pH

6.7 (10 g/L)

mp

>360 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Nc1c(cc(c2ccccc12)S([O-])(=O)=O)\N=N\c3ccc(cc3)-c4ccc(cc4)\N=N\c5cc(c6ccccc6c5N)S([O-])(=O)=O

InChI

1S/C32H24N6O6S2.2Na/c33-31-25-7-3-1-5-23(25)29(45(39,40)41)17-27(31)37-35-21-13-9-19(10-14-21)20-11-15-22(16-12-20)36-38-28-18-30(46(42,43)44)24-6-2-4-8-26(24)32(28)34;;/h1-18H,33-34H2,(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2/b37-35+,38-36+;;

InChI key

IQFVPQOLBLOTPF-HKXUKFGYSA-L

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This Item
C6277N700575768
Congo Red Dye content ≥35 %

Sigma-Aldrich

C6767

Congo Red

Congo Red certified by the Biological Stain Commission, BioXtra

Sigma-Aldrich

C6277

Congo Red

Neutral Red Dye content ≥90 %

Sigma-Aldrich

N7005

Neutral Red

Congo Red analytical standard

Supelco

75768

Congo Red

composition

Dye content, ≥35%

composition

Dye content, ≥85%

composition

Dye content, ≥90%

composition

-

technique(s)

titration: suitable

technique(s)

-

technique(s)

titration: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

pH

6.7 (10 g/L)

pH

-

pH

6.8-8.0, red to yellow

pH

-

mp

>360 °C (lit.)

mp

>360 °C (lit.)

mp

290 °C (dec.) (lit.)

mp

>360 °C (lit.)

solubility

H2O: 1 mg/mL

solubility

H2O: 10 mg/mL

solubility

water: 10 mg/mL, dark red

solubility

-

Application

  • Congo red has been used as a histochemical stain for the quantification of amyloid deposits in brain tissues.
  • It has been used as a cell wall inhibiting agent in fungus Cryptococcus neoformans.
  • It has also been used to study microstructural details in the cell walls of raw potato and fried potato using confocal microscopy.

Biochem/physiol Actions

Congo red is a benzidine-based anionic diazo dye. It is a histological dye which binds to many amyloid proteins and is used for the quantification of amyloid β-peptide aggregation. Congo red also interacts with β-D-glucans, polysaccharides containing continuous β-(1→4)-linked D-glucopyranosyl units and some hemicellulosic galactoglucomannans.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Repr. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain.
Wilcock DM, et al.
Nature Protocols, 1, 1591-1591 (2006)
Kinetic study and equilibrium isotherm analysis of Congo Red adsorption by clay materials.
Chemical Engineering Journal, 148, 354-364 (2009)
PKC1 is essential for protection against both oxidative and nitrosative stresses, cell integrity, and normal manifestation of virulence factors in the pathogenic fungus Cryptococcus neoformans.
Gerik KJ, et al.
Eukaryotic Cell, 7, 1685-1685 (2008)
Anat Frydman-Marom et al.
PloS one, 6(1), e16564-e16564 (2011-02-10)
An increasing body of evidence indicates that accumulation of soluble oligomeric assemblies of β-amyloid polypeptide (Aβ) play a key role in Alzheimer's disease (AD) pathology. Specifically, 56 kDa oligomeric species were shown to be correlated with impaired cognitive function in
Quantifying amyloid beta-peptide (Abeta) aggregation using the Congo red-Abeta (CR-abeta) spectrophotometric assay.
Klunk WE, et al.
Analytical Biochemistry, 266, 66-66 (1999)

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